Metabolic responses to prolonged starvation, food restriction, and refeeding in the brown trout, Salmo trutta: Oxidative stress and antioxidant defenses

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2011-08, Vol.159 (4), p.191-196
• Main Authors: Bayir, Abdulkadir, Sirkecioglu, A. Necdet, Bayir, Mehtap, Haliloglu, H. Ibrahim, Kocaman, E. Mahmut, Aras, N. Mevlut
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant defenses; antioxidants; Antioxidants - metabolism; Body Weight; catalase; enzyme activity; Feeding Behavior; fish; food deprivation; gills; Gills - enzymology; Gills - metabolism; glucose-6-phosphate 1-dehydrogenase; glutathione peroxidase; glutathione transferase; glutathione-disulfide reductase; Lipid Peroxidation; liver; Liver - enzymology; Liver - metabolism; Liver - pathology; long term effects; malondialdehyde; normal values; Organ Size; Oxidative Stress; Refeeding; Salmo trutta; Salmonidae - anatomy & histology; Salmonidae - metabolism; Starvation; Starvation - metabolism; Starvation - pathology; Starvation - physiopathology; superoxide dismutase
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2011.04.008

The effects of long-term starvation and food restriction (49 days), followed by refeeding (21 days) have been studied with respect to antioxidant defense in the liver and gills (branchial tissues) of the brown trout, Salmo trutta. Malondialdehyde levels in both tissues increased in parallel with starvation and food restriction and these values did not return to normal after the refeeding period. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in liver and gills increased during the 49 days of starvation, but glucose-6-phosphate dehydrogenase (G6PD) activities decreased. Glutathione S-transferase (GST) activity decreased in the liver at the 49th day of starvation, but increased in the branchial tissues. Some of the antioxidant enzyme activities (such as hepatic GST and branchial G6PD) returned to control values of fed fish after the refeeding period, but others (e.g. hepatic SOD and branchial GPx) did not return to normal values. In conclusion, our study indicates that total or partial food deprivation induces oxidative stress in brown trout.