Engineering of the Redox Ribozyme for the Determination of Its Architecture

Here we report engineering efforts on an artificially evolved redox ribozyme, known as ribox02, and its more compact pseudoknot construct generated, combox02. The rigorous mutational works in the various regions suggested that the catalytically essential domain composed of three joining regions flan...

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Bibliographic Details
Published in:Chemistry letters 2010-07, Vol.39 (7), p.786-787
Main Authors: Pattnaik, Swetansu. B, Jin, Koichiro, Futai, Kazuki, Suga, Hiroaki
Format: Article
Language:English
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Summary:Here we report engineering efforts on an artificially evolved redox ribozyme, known as ribox02, and its more compact pseudoknot construct generated, combox02. The rigorous mutational works in the various regions suggested that the catalytically essential domain composed of three joining regions flanked by three stems. The architecture of combox02 can be an excellent framework for constructing libraries by randomizing sequences in this region for in vitro selection of novel ribozymes capable of catalyzing various chemical conversions of 5′-substrates.
ISSN:0366-7022
1348-0715
1348-0715
DOI:10.1246/cl.2010.786