Molecular characterization and expression patterns of Lbx1 in porcine skeletal muscle

• Published in: Molecular biology reports 2011-08, Vol.38 (6), p.3983-3991
• Main Authors: Chao, Zhe, Wu, Jian, Zheng, Rong, Li, Feng-E., Xiong, Yuan-Zhu, Deng, Chang-Yan
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animal Anatomy; Animal Biochemistry; Animals; Biomedical and Life Sciences; Gene Expression Profiling; Gene Expression Regulation; Gene Frequency - genetics; Genetic Association Studies; Genotype; Histology; Life Sciences; Meat - economics; Molecular biology; Molecular Sequence Data; Morphology; Muscle Development - genetics; Muscle Proteins - chemistry; Muscle Proteins - genetics; Muscle Proteins - metabolism; Muscle, Skeletal - growth & development; Muscle, Skeletal - metabolism; Muscular system; Phylogeny; Polymorphism, Restriction Fragment Length - genetics; Polymorphism, Single Nucleotide - genetics; Quantitative Trait, Heritable; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA; Skeletal system; Sus scrofa - genetics; Sus scrofa - growth & development
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-010-0516-1

Ladybird -like genes were recently identified in mammals. The first member characterized, Lbx1 , is expressed in developing skeletal muscle and the nervous system. However, little is known about the porcine Lbx1 gene. In the present study, we cloned and characterized Lbx1 from porcine muscle. RT-PCR analyses showed that Lbx1 was highly expressed in porcine skeletal muscle tissues. And we provide the first evidence that Lbx1 has a certain regulated expression pattern during the postnatal period of the porcine skeletal muscle development. Lbx1 gene expressed at higher levels in biceps femoris muscles compared with masseter , semitendinosus and longissimus dorsi muscles in Meishan pigs. Phylogenetic tree was constructed by aligning the amino acid sequences of different species. Moreover, single nucleotide polymorphism (SNP) scanning in the Lbx1 genomic fragment identified two mutations, g.752A>G and g.−1559C>G. Association analysis in our experimental pig populations showed that the mutation of g.752A>G was significantly associated with loin muscle area ( P