Molecular detection of Anaplasma phagocytophilum in cattle and Ixodes persulcatus ticks

The tick-borne pathogen, Anaplasma phagocytophilum (A. phagocytophilum), the causative agent of human granulocytic anaplasmaposis (HGA), is increasingly becoming a public health concern as an aetiological agent for emerging infectious disease. We found A. phagocytophilum infection in a pooled sample...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary microbiology 2011-05, Vol.149 (3-4), p.504-507
Main Authors: Murase, Yusuke, Konnai, Satoru, Hidano, Arata, Githaka, Naftali W., Ito, Takuya, Takano, Ai, Kawabata, Hiroki, Ato, Manabu, Tajima, Tomoko, Tajima, Motoshi, Onuma, Misao, Murata, Shiro, Ohashi, Kazuhiko
Format: Article
Language:English
Subjects:
Anaplasma phagocytophilum
Anaplasma phagocytophilum - genetics
Anaplasma phagocytophilum - isolation & purification
Anaplasmosis - epidemiology
Anaplasmosis - microbiology
Animals
Arthropod Vectors - microbiology
Bacteriology
Biological and medical sciences
Cattle
Cattle - microbiology
Cattle Diseases - epidemiology
Cattle Diseases - microbiology
DNA
DNA, Bacterial - genetics
emerging diseases
Female
Fundamental and applied biological sciences. Psychology
genes
humans
Ixodes - microbiology
Ixodes persulcatus
Ixodidae
Japan - epidemiology
Male
Microbiology
Miscellaneous
outer membrane proteins
pastures
pathogenesis
pathogens
Polymerase Chain Reaction
Prevalence
public health
Sequence Analysis, DNA
Tick
ticks
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The tick-borne pathogen, Anaplasma phagocytophilum (A. phagocytophilum), the causative agent of human granulocytic anaplasmaposis (HGA), is increasingly becoming a public health concern as an aetiological agent for emerging infectious disease. We found A. phagocytophilum infection in a pooled sample of field-collected Ixodes persulcatus (I. persulcatus) ticks from one district in Hokkaido, Japan. Thus, to further investigate the prevalence in field-collected ticks, we used PCR assays targeting the A. phagocytophilum gene encoding 44kDa major outer membrane protein (p44) for screening of I. persulcatus ticks and samples from cattle from pastures. Out of the 281 I. persulcatus ticks, 20 (7.1%) were found to harbor A. phagocytophilum DNA. The infection rate for A. phagocytophilum in cattle was 3.4% (42/1251). In future studies, it will be necessary to investigate effects of the infection in order to understand its pathogenesis of A. phagocytophilum in domestic animals.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2010.11.025