Effects of nutrient dosing on subsurface methanotrophic populations and trichloroethylene degradation

In in situ bioremediation demonstration at the Savannah River Site in Aiken, South Carolina, trichloroethylene-degrading microorganisms were stimulated by delivering nutrients to the TCE-contaminated subsurface via horizontal injection wells. Microbial and chemical monitoring of groundwater from 12...

Full description

Saved in:
Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 1997-02, Vol.18 (2/3), p.204-212
Main Authors: Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.), Palumbo, A.V, Phelps, T.J, Hazen, T.C
Format: Article
Language:English
Subjects:
BIODECONTAMINACION
Biodegradation, Environmental
BIOREMEDIATION
Chlorides - metabolism
Chlorides - pharmacology
Culture Media - pharmacology
Environmental Pollutants - metabolism
Environmental Pollution
Geologic Sediments - analysis
Groundwater
HIDROCARBUROS HALOGENADOS
HYDROCARBURE HALOGENE
Injection
Injection wells
Methane
Methane - analysis
Methane - metabolism
Methane - pharmacology
Methanomicrobiales - metabolism
Microbial activity
Microorganisms
Nitrogen
Nitrogen - analysis
Nitrogen - metabolism
Nitrogen - pharmacology
Nutrients
Phosphorus - analysis
Phosphorus - metabolism
Phosphorus - pharmacology
Solvents
Trichloroethene
Trichloroethylene
Trichloroethylene - analysis
Trichloroethylene - metabolism
Water Microbiology
Water monitoring
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c471t-bf9aa2b2643eabc12102794f4905f77ebdb16a601e8e3333cdd220dbcfe0c0fc3
cites
container_end_page 212
container_issue 2/3
container_start_page 204
container_title Journal of industrial microbiology & biotechnology
container_volume 18
creator Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.)
Palumbo, A.V
Phelps, T.J
Hazen, T.C
description In in situ bioremediation demonstration at the Savannah River Site in Aiken, South Carolina, trichloroethylene-degrading microorganisms were stimulated by delivering nutrients to the TCE-contaminated subsurface via horizontal injection wells. Microbial and chemical monitoring of groundwater from 12 vertical wells was used to examine the effects of methane and nutrient (nitrogen and phosphorus) dosing on the methanotrophic populations and or the potential of the subsurface microbial communities to degrade TCE. Densities of methanotrophs increased 3-5 orders of magnitude during the methane- and nutrient-injection phases; this increase coincided with the higher methane levels observed in the monitoring wells. TCE degradation capacity, although not directly tied to methane concentration, responded to the methane injection, and responded more dramatically to the multiple-nutrient injection. These results support the crucial role of methane, nitrogen, and phosphorus as amended nutrients in TCE bioremediation. The enhancing effects of nutrient dosing on microbial abundance and degradative potentials, coupled with increased chloride concentrations, provided multiple lines of evidence substantiating the effectiveness of this integrated in situ bioremediation process
doi_str_mv 10.1038/sj.jim.2900350
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_872129029</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2186094611</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-bf9aa2b2643eabc12102794f4905f77ebdb16a601e8e3333cdd220dbcfe0c0fc3</originalsourceid><addsrcrecordid>eNqN0b1r3DAYBnBRGtIk7dqhUBAZkslXfVk6j-XIFwQyJJmFLL26s7ElV7KH_PdV7o4OHUq1SPD-9ID0IPSVkhUlfP0j96u-G1esIYTX5AM6o0LJqq55_bGcuVRVLXj9CZ3n3BNCaqXYKTptKC9MnSG48R7snHH0OCxz6iDM2MXchS2OAeelzUvyxgIeYd6ZEOcUp11n8RSnZTBzF0PGJjhcrtrdEFMs7G2AANjBNhm3J5_RiTdDhi_H_QK93t68bO6rx6e7h83Px8oKReeq9Y0xrGVScDCtpYwSphrhRUNqrxS0rqXSSEJhDbws6xxjxLXWA7HEW36Brg-5U4q_FsizHrtsYRhMgLhkvVaMlp9izf9JIYUs8uqfkkrC1kK-R17-Bfu4pFDeq5XkXFG1T1sdkE0x5wReT6kbTXrTlOj3QnXudSlUHwstF74fU5d2BPeHHxss82-HuTdRm23qsn59bhRnvIjf6FOmzA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>763371746</pqid></control><display><type>article</type><title>Effects of nutrient dosing on subsurface methanotrophic populations and trichloroethylene degradation</title><source>ABI/INFORM Global</source><source>Oxford Open</source><creator>Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.) ; Palumbo, A.V ; Phelps, T.J ; Hazen, T.C</creator><creatorcontrib>Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.) ; Palumbo, A.V ; Phelps, T.J ; Hazen, T.C</creatorcontrib><description>In in situ bioremediation demonstration at the Savannah River Site in Aiken, South Carolina, trichloroethylene-degrading microorganisms were stimulated by delivering nutrients to the TCE-contaminated subsurface via horizontal injection wells. Microbial and chemical monitoring of groundwater from 12 vertical wells was used to examine the effects of methane and nutrient (nitrogen and phosphorus) dosing on the methanotrophic populations and or the potential of the subsurface microbial communities to degrade TCE. Densities of methanotrophs increased 3-5 orders of magnitude during the methane- and nutrient-injection phases; this increase coincided with the higher methane levels observed in the monitoring wells. TCE degradation capacity, although not directly tied to methane concentration, responded to the methane injection, and responded more dramatically to the multiple-nutrient injection. These results support the crucial role of methane, nitrogen, and phosphorus as amended nutrients in TCE bioremediation. The enhancing effects of nutrient dosing on microbial abundance and degradative potentials, coupled with increased chloride concentrations, provided multiple lines of evidence substantiating the effectiveness of this integrated in situ bioremediation process</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1038/sj.jim.2900350</identifier><identifier>PMID: 9134767</identifier><language>eng</language><publisher>Germany: Oxford University Press</publisher><subject>BIODECONTAMINACION ; Biodegradation, Environmental ; BIOREMEDIATION ; Chlorides - metabolism ; Chlorides - pharmacology ; Culture Media - pharmacology ; Environmental Pollutants - metabolism ; Environmental Pollution ; Geologic Sediments - analysis ; Groundwater ; HIDROCARBUROS HALOGENADOS ; HYDROCARBURE HALOGENE ; Injection ; Injection wells ; Methane ; Methane - analysis ; Methane - metabolism ; Methane - pharmacology ; Methanomicrobiales - metabolism ; Microbial activity ; Microorganisms ; Nitrogen ; Nitrogen - analysis ; Nitrogen - metabolism ; Nitrogen - pharmacology ; Nutrients ; Phosphorus - analysis ; Phosphorus - metabolism ; Phosphorus - pharmacology ; Solvents ; Trichloroethene ; Trichloroethylene ; Trichloroethylene - analysis ; Trichloroethylene - metabolism ; Water Microbiology ; Water monitoring</subject><ispartof>Journal of industrial microbiology &amp; biotechnology, 1997-02, Vol.18 (2/3), p.204-212</ispartof><rights>Society for Industrial Microbiology 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-bf9aa2b2643eabc12102794f4905f77ebdb16a601e8e3333cdd220dbcfe0c0fc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/763371746/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/763371746?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,11668,27903,27904,36039,36040,44342,74641</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9134767$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.)</creatorcontrib><creatorcontrib>Palumbo, A.V</creatorcontrib><creatorcontrib>Phelps, T.J</creatorcontrib><creatorcontrib>Hazen, T.C</creatorcontrib><title>Effects of nutrient dosing on subsurface methanotrophic populations and trichloroethylene degradation</title><title>Journal of industrial microbiology &amp; biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><description>In in situ bioremediation demonstration at the Savannah River Site in Aiken, South Carolina, trichloroethylene-degrading microorganisms were stimulated by delivering nutrients to the TCE-contaminated subsurface via horizontal injection wells. Microbial and chemical monitoring of groundwater from 12 vertical wells was used to examine the effects of methane and nutrient (nitrogen and phosphorus) dosing on the methanotrophic populations and or the potential of the subsurface microbial communities to degrade TCE. Densities of methanotrophs increased 3-5 orders of magnitude during the methane- and nutrient-injection phases; this increase coincided with the higher methane levels observed in the monitoring wells. TCE degradation capacity, although not directly tied to methane concentration, responded to the methane injection, and responded more dramatically to the multiple-nutrient injection. These results support the crucial role of methane, nitrogen, and phosphorus as amended nutrients in TCE bioremediation. The enhancing effects of nutrient dosing on microbial abundance and degradative potentials, coupled with increased chloride concentrations, provided multiple lines of evidence substantiating the effectiveness of this integrated in situ bioremediation process</description><subject>BIODECONTAMINACION</subject><subject>Biodegradation, Environmental</subject><subject>BIOREMEDIATION</subject><subject>Chlorides - metabolism</subject><subject>Chlorides - pharmacology</subject><subject>Culture Media - pharmacology</subject><subject>Environmental Pollutants - metabolism</subject><subject>Environmental Pollution</subject><subject>Geologic Sediments - analysis</subject><subject>Groundwater</subject><subject>HIDROCARBUROS HALOGENADOS</subject><subject>HYDROCARBURE HALOGENE</subject><subject>Injection</subject><subject>Injection wells</subject><subject>Methane</subject><subject>Methane - analysis</subject><subject>Methane - metabolism</subject><subject>Methane - pharmacology</subject><subject>Methanomicrobiales - metabolism</subject><subject>Microbial activity</subject><subject>Microorganisms</subject><subject>Nitrogen</subject><subject>Nitrogen - analysis</subject><subject>Nitrogen - metabolism</subject><subject>Nitrogen - pharmacology</subject><subject>Nutrients</subject><subject>Phosphorus - analysis</subject><subject>Phosphorus - metabolism</subject><subject>Phosphorus - pharmacology</subject><subject>Solvents</subject><subject>Trichloroethene</subject><subject>Trichloroethylene</subject><subject>Trichloroethylene - analysis</subject><subject>Trichloroethylene - metabolism</subject><subject>Water Microbiology</subject><subject>Water monitoring</subject><issn>1367-5435</issn><issn>1476-5535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>M0C</sourceid><recordid>eNqN0b1r3DAYBnBRGtIk7dqhUBAZkslXfVk6j-XIFwQyJJmFLL26s7ElV7KH_PdV7o4OHUq1SPD-9ID0IPSVkhUlfP0j96u-G1esIYTX5AM6o0LJqq55_bGcuVRVLXj9CZ3n3BNCaqXYKTptKC9MnSG48R7snHH0OCxz6iDM2MXchS2OAeelzUvyxgIeYd6ZEOcUp11n8RSnZTBzF0PGJjhcrtrdEFMs7G2AANjBNhm3J5_RiTdDhi_H_QK93t68bO6rx6e7h83Px8oKReeq9Y0xrGVScDCtpYwSphrhRUNqrxS0rqXSSEJhDbws6xxjxLXWA7HEW36Brg-5U4q_FsizHrtsYRhMgLhkvVaMlp9izf9JIYUs8uqfkkrC1kK-R17-Bfu4pFDeq5XkXFG1T1sdkE0x5wReT6kbTXrTlOj3QnXudSlUHwstF74fU5d2BPeHHxss82-HuTdRm23qsn59bhRnvIjf6FOmzA</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.)</creator><creator>Palumbo, A.V</creator><creator>Phelps, T.J</creator><creator>Hazen, T.C</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7QO</scope><scope>7TV</scope></search><sort><creationdate>19970201</creationdate><title>Effects of nutrient dosing on subsurface methanotrophic populations and trichloroethylene degradation</title><author>Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.) ; Palumbo, A.V ; Phelps, T.J ; Hazen, T.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-bf9aa2b2643eabc12102794f4905f77ebdb16a601e8e3333cdd220dbcfe0c0fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>BIODECONTAMINACION</topic><topic>Biodegradation, Environmental</topic><topic>BIOREMEDIATION</topic><topic>Chlorides - metabolism</topic><topic>Chlorides - pharmacology</topic><topic>Culture Media - pharmacology</topic><topic>Environmental Pollutants - metabolism</topic><topic>Environmental Pollution</topic><topic>Geologic Sediments - analysis</topic><topic>Groundwater</topic><topic>HIDROCARBUROS HALOGENADOS</topic><topic>HYDROCARBURE HALOGENE</topic><topic>Injection</topic><topic>Injection wells</topic><topic>Methane</topic><topic>Methane - analysis</topic><topic>Methane - metabolism</topic><topic>Methane - pharmacology</topic><topic>Methanomicrobiales - metabolism</topic><topic>Microbial activity</topic><topic>Microorganisms</topic><topic>Nitrogen</topic><topic>Nitrogen - analysis</topic><topic>Nitrogen - metabolism</topic><topic>Nitrogen - pharmacology</topic><topic>Nutrients</topic><topic>Phosphorus - analysis</topic><topic>Phosphorus - metabolism</topic><topic>Phosphorus - pharmacology</topic><topic>Solvents</topic><topic>Trichloroethene</topic><topic>Trichloroethylene</topic><topic>Trichloroethylene - analysis</topic><topic>Trichloroethylene - metabolism</topic><topic>Water Microbiology</topic><topic>Water monitoring</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.)</creatorcontrib><creatorcontrib>Palumbo, A.V</creatorcontrib><creatorcontrib>Phelps, T.J</creatorcontrib><creatorcontrib>Hazen, T.C</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>ProQuest Business Premium Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Biological Sciences</collection><collection>ABI/INFORM Global</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>One Business (ProQuest)</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Pollution Abstracts</collection><jtitle>Journal of industrial microbiology &amp; biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pfiffner, S.M. (Oak Ridge National Laboratory, Oak Ridge, TN.)</au><au>Palumbo, A.V</au><au>Phelps, T.J</au><au>Hazen, T.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of nutrient dosing on subsurface methanotrophic populations and trichloroethylene degradation</atitle><jtitle>Journal of industrial microbiology &amp; biotechnology</jtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>18</volume><issue>2/3</issue><spage>204</spage><epage>212</epage><pages>204-212</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>In in situ bioremediation demonstration at the Savannah River Site in Aiken, South Carolina, trichloroethylene-degrading microorganisms were stimulated by delivering nutrients to the TCE-contaminated subsurface via horizontal injection wells. Microbial and chemical monitoring of groundwater from 12 vertical wells was used to examine the effects of methane and nutrient (nitrogen and phosphorus) dosing on the methanotrophic populations and or the potential of the subsurface microbial communities to degrade TCE. Densities of methanotrophs increased 3-5 orders of magnitude during the methane- and nutrient-injection phases; this increase coincided with the higher methane levels observed in the monitoring wells. TCE degradation capacity, although not directly tied to methane concentration, responded to the methane injection, and responded more dramatically to the multiple-nutrient injection. These results support the crucial role of methane, nitrogen, and phosphorus as amended nutrients in TCE bioremediation. The enhancing effects of nutrient dosing on microbial abundance and degradative potentials, coupled with increased chloride concentrations, provided multiple lines of evidence substantiating the effectiveness of this integrated in situ bioremediation process</abstract><cop>Germany</cop><pub>Oxford University Press</pub><pmid>9134767</pmid><doi>10.1038/sj.jim.2900350</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1367-5435
ispartof Journal of industrial microbiology & biotechnology, 1997-02, Vol.18 (2/3), p.204-212
issn 1367-5435
1476-5535
language eng
recordid cdi_proquest_miscellaneous_872129029
source ABI/INFORM Global; Oxford Open
subjects BIODECONTAMINACION
Biodegradation, Environmental
BIOREMEDIATION
Chlorides - metabolism
Chlorides - pharmacology
Culture Media - pharmacology
Environmental Pollutants - metabolism
Environmental Pollution
Geologic Sediments - analysis
Groundwater
HIDROCARBUROS HALOGENADOS
HYDROCARBURE HALOGENE
Injection
Injection wells
Methane
Methane - analysis
Methane - metabolism
Methane - pharmacology
Methanomicrobiales - metabolism
Microbial activity
Microorganisms
Nitrogen
Nitrogen - analysis
Nitrogen - metabolism
Nitrogen - pharmacology
Nutrients
Phosphorus - analysis
Phosphorus - metabolism
Phosphorus - pharmacology
Solvents
Trichloroethene
Trichloroethylene
Trichloroethylene - analysis
Trichloroethylene - metabolism
Water Microbiology
Water monitoring
title Effects of nutrient dosing on subsurface methanotrophic populations and trichloroethylene degradation
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T17%3A07%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20nutrient%20dosing%20on%20subsurface%20methanotrophic%20populations%20and%20trichloroethylene%20degradation&rft.jtitle=Journal%20of%20industrial%20microbiology%20&%20biotechnology&rft.au=Pfiffner,%20S.M.%20(Oak%20Ridge%20National%20Laboratory,%20Oak%20Ridge,%20TN.)&rft.date=1997-02-01&rft.volume=18&rft.issue=2/3&rft.spage=204&rft.epage=212&rft.pages=204-212&rft.issn=1367-5435&rft.eissn=1476-5535&rft_id=info:doi/10.1038/sj.jim.2900350&rft_dat=%3Cproquest_cross%3E2186094611%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c471t-bf9aa2b2643eabc12102794f4905f77ebdb16a601e8e3333cdd220dbcfe0c0fc3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=763371746&rft_id=info:pmid/9134767&rfr_iscdi=true