Effect of centrifugation and sugar supplementation on the semen cryopreservation of captive collared peccaries ( Tayassu tajacu)

• Published in: Cryobiology 2010-12, Vol.61 (3), p.275-279
• Main Authors: Castelo, T.S., Bezerra, F.S.B., Lima, G.L., Alves, H.M., Oliveira, I.R.S., Santos, E.A.A., Peixoto, G.C.X., Silva, A.R.
• Format: Article
• Language: English
• Subjects: Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Centrifugation; Collared peccary; Conservation, protection and management of environment and wildlife; Cryopreservation - methods; Cryopreservation - veterinary; Diverse techniques; Fructose - pharmacology; Fundamental and applied biological sciences. Psychology; Glucose - pharmacology; Humans; Male; Mammals; Molecular and cellular biology; Semen; Semen - chemistry; Semen Preservation - methods; Semen Preservation - veterinary; Seminal plasma; Sperm Motility; Spermatozoa - drug effects; Sugar; Tayassu tajacu
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2010.09.005

The present study is aimed at evaluating the effect of centrifugation for seminal plasma removal and the supplementation of fructose or glucose to the Tris-based extender on the kinematic patterns of the motility parameters of frozen–thawed semen obtained from captive collared peccaries ( Tayassu tajacu). Semen samples ( n = 14) were collected from 10 sexually mature male collared peccaries by electroejaculation. These samples were further evaluated for parameters such as motility, vigor, sperm viability, membrane integrity, and sperm morphology. The samples were divided into four aliquots, and only two of these aliquots were centrifuged. The semen aliquots (centrifuged and raw semen samples) were diluted in Tris-based extenders supplemented with fructose or glucose. Egg yolk (20%) and glycerol (3%) were added to all the samples which were cryopreserved in liquid nitrogen and thawed at 37 °C/1 min. The frozen–thawed semen was evaluated for the same parameters described for the fresh semen. On the other hand, the kinematic motility patterns were evaluated by a computer-aided system. After thawing, it was observed that the values for the total sperm motility were around 30% for all the samples. A negative effect of centrifugation was verified for parameters such as sperm morphology, linearity, straightness, and beat cross frequency ( P < 0.05). However, no differences between fructose and glucose were verified for any semen end point ( P > 0.05). In conclusion, it is not recommended to centrifuge the ejaculates from collared peccaries prior to conducting the cryopreservative procedures using a Tris-based extender supplemented with fructose or glucose.