Characterization of Phages Virulent for Robinia pseudoacacia Rhizobia

Three lytic phages (ΦRP1, ΦRP2, and ΦRP3) specific for Robinia pseudoacacia rhizobia were isolated from the soil under black locust. They were characterized by their morphology, host range, and some other properties including DNA molecular weights. Studied phages have been found to belong to Siphovi...

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Bibliographic Details
Published in:Current microbiology 2009-08, Vol.59 (2), p.187-192
Main Authors: Małek, Wanda, Wdowiak-Wróbel, Sylwia, Bartosik, Michał, Konopa, Grażyna, Narajczyk, Magdalena
Format: Article
Language:English
Subjects:
Bacteria
Bacteriophages - genetics
Bacteriophages - growth & development
Bacteriophages - isolation & purification
Bacteriophages - ultrastructure
Biomedical and Life Sciences
Biotechnology
Burst size
DNA
Enzymes
Host range
Life Sciences
Microbiology
Molecular Weight
Morphology
Phages
Rhizobiaceae - virology
Robinia - microbiology
Robinia pseudoacacia
Siphoviridae
Siphoviridae - ultrastructure
Soil
Soil Microbiology
Soil microorganisms
Tails
Virion - ultrastructure
Virus Attachment
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Summary:Three lytic phages (ΦRP1, ΦRP2, and ΦRP3) specific for Robinia pseudoacacia rhizobia were isolated from the soil under black locust. They were characterized by their morphology, host range, and some other properties including DNA molecular weights. Studied phages have been found to belong to Siphoviridae family that comprises viruses with long, and noncontractile tails. They had broad host ranges and effectively lysed not only Robinia pseudoacacia microsymbionts but also different Mesorhizobium species. The phages were homogenous in latent periods (300 min) but heterogeneous in burst sizes (100-200 phage particles per one infected cell) and rise periods (90-120 min). They showed a distinct adsorption rate to Robinia pseudoacacia rhizobia (70.4-93.94%). The molecular weights of phage DNAs estimated from restriction enzyme digests were in the range from ca. 82 kb to ca. 105 kb.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-009-9421-z