Isolation and Characterization of a New Benzene, Toluene, and Ethylbenzene Degrading Bacterium, Acinetobacter sp. B113

A bacterium designated strain B113, able to degrade benzene, toluene, and ethylbenzene compounds (BTE), was isolated from gasoline-contaminated sediment at a gas station in Geoje, Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Acinetobacte...

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Bibliographic Details
Published in:Current microbiology 2009-01, Vol.58 (1), p.70-75
Main Authors: Kim, Jeong Myeong, Jeon, Che Ok
Format: Article
Language:English
Subjects:
Acinetobacter
Acinetobacter - classification
Acinetobacter - isolation & purification
Acinetobacter - metabolism
Bacteria
Benzene
Benzene - metabolism
Benzene Derivatives - metabolism
Biodegradation
Biomedical and Life Sciences
Biotechnology
Biotransformation
Contaminated sediments
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Gasoline
Genes, rRNA
Genetics
Korea
Life Sciences
Microbiology
Molecular Sequence Data
Organic compounds
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Sediment pollution
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Slurries
Soil contaminants
Soil Microbiology
Toluene
Toluene - metabolism
Yeast
Yeasts
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Summary:A bacterium designated strain B113, able to degrade benzene, toluene, and ethylbenzene compounds (BTE), was isolated from gasoline-contaminated sediment at a gas station in Geoje, Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Acinetobacter. The biodegradation rates of benzene, toluene, and ethylbenzene were relatively low in MSB broth, but the addition of yeast extract had a substantial impact on the biodegradation of BTE compounds, which suggested that yeast extract might provide a factor that was necessary for its growth or BTE biodegradation activity. However, interestingly, the biodegradation of BTE compounds occurred very quickly in slurry systems amended with sterile soil. Moreover, if soil was combusted first to remove organic matters, the enhancement effect on BTE biodegradation was lost, indicating that some insoluble organic compounds were probably beneficial for BTE degradation in contaminated sediment. This study suggests that strain B113 may play an important role for biodegradation of BTE in the contaminated site.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-008-9268-8