Pentagalloylglucose downregulates cofilin1 and inhibits HSV-1 infection

To investigate the anti-herpesvirus mechanism of pentagalloylglucose (PGG), we compared the proteomic changes between herpes simplex virus type 1 (HSV-1) infected MRC-5 cells with or without PGG-treatment, and between non-infected MRC-5 cells with or without PGG-treatment by 2-DE and MS-based analys...

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Bibliographic Details
Published in:Antiviral research 2011, Vol.89 (1), p.98-108
Main Authors: Pei, Ying, Xiang, Yang-Fei, Chen, Jia-Nan, Lu, Chun-Hua, Hao, Jing, Du, Qian, lai, Chi-choi, Qu, Chang, Li, Shen, Ju, Huai-Qiang, Ren, Zhe, Liu, Qiu-Ying, Xiong, Sheng, Qian, Chui-Wen, Zeng, Fan-Li, Zhang, Pei-Zhuo, Yang, Chong-Ren, Zhang, Ying-Jun, Xu, Jun, Kitazato, Kaio, Wang, Yi-Fei
Format: Article
Language:English
Subjects:
Actin cytoskeleton
Animals
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiviral agents
Biological and medical sciences
Cell Line
Cercopithecus aethiops
Cofilin 1 - antagonists & inhibitors
Cofilin 1 - biosynthesis
Cofilin 1 - genetics
Cofilin1
Down-Regulation
Gene Knockdown Techniques
Herpes simplex virus 1
Herpes simplex virus type 1
Herpesvirus 1, Human - physiology
Humans
Hydrolyzable Tannins - metabolism
Medical sciences
Pentagalloylglucose
Pharmacology. Drug treatments
RNA, Small Interfering - metabolism
Two-dimensional gel electrophoresis
Virus Internalization - drug effects
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Summary:To investigate the anti-herpesvirus mechanism of pentagalloylglucose (PGG), we compared the proteomic changes between herpes simplex virus type 1 (HSV-1) infected MRC-5 cells with or without PGG-treatment, and between non-infected MRC-5 cells with or without PGG-treatment by 2-DE and MS-based analysis. Differentially expressed cellular proteins were mainly involved with actin cytoskeleton regulation. Significantly, PGG can down-regulate cofilin1, a key regulator of actin cytoskeleton dynamics. PGG can inhibit HSV-1-induced rearrangements of actin cytoskeleton which is important for infectivity. Furthermore, cofilin1 knockdown by siRNA also inhibited the HSV-1-induced actin-skeleton rearrangements. Both PGG-treatment and cofilin1 knockdown can reduce HSV-1 DNA, mRNA, protein synthesis and virus yields. Altogether, the results suggested that down-regulating cofilin1 plays a role in PGG inhibiting HSV-1 infection. PGG may be a promising anti-herpesvirus agent for drug development.
ISSN:0166-3542
1872-9096
DOI:10.1016/j.antiviral.2010.11.012