Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers

Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between acces...

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Bibliographic Details
Published in:Genetics and molecular research 2010-01, Vol.9 (3), p.1673-1682
Main Authors: Nawaz, S, Khan, F A, Tabasum, S, Zakria, M, Saeed, A, Iqbal, M Z
Format: Article
Language:English
Subjects:
DNA, Plant - genetics
Genetic Variation - genetics
Genotype
Pakistan
Phylogeny
Polymerase Chain Reaction
Random Amplified Polymorphic DNA Technique - methods
Saccharum
Saccharum - classification
Saccharum - genetics
Saccharum officinarum
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Summary:Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dendrogram. Genetic distance values ranging from 16.2 to 86.3% were observed among the 40 sugarcane accessions. The lowest genetic distance was found between genotypes US-406 and US-186. These two genotypes differed from each other in only 25 bands with 15 different primers. Genotypes Col-54 and CP-72-2086 were the second most similar group, with a genetic distance of 19.46%. The most dissimilar of all the accessions were CP-77-400 and US-133, with a genetic distance of 86.3%. RAPD fingerprints help sugarcane breeders clarify the genetic pedigree of commercial sugarcane varieties and can be used to evaluate the efficiency of conventional breeding methods.
ISSN:1676-5680
1676-5680
DOI:10.4238/vol9-3gmr903