Identification of volatiles from pineapple (Ananas comosus L.) pulp by comprehensive two-dimensional gas chromatography and gas chromatography/mass spectrometry

Combining qualitative data from the chromatographic structure of 2-D gas chromatography with flame ionization detection (GC×GC-FID) and that from gas chromatography-mass spectrometry (GC/MS) should result in a more accurate assignment of the peak identities than the simple analysis by GC/MS, where c...

Full description

Saved in:
Bibliographic Details
Published in:Journal of separation science 2011-07, Vol.34 (13), p.1547-1554
Main Authors: Pedroso, Marcio P, Ferreira, Ernesto C, Hantao, Leandro W, Bogusz, Stanislau Jr, Augusto, Fabio
Format: Article
Language:English
Subjects:
Ananas - chemistry
Ananas comosus
Biological and medical sciences
Chromatographic structure
Comprehensive 2-D gas chromatography
Computer programs
computer software
Deconvolution
Extraction
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Gas chromatography
Gas chromatography-mass spectrometry
Gas Chromatography-Mass Spectrometry - methods
headspace analysis
ionization
Mass spectra
mass spectrometry
Matrices
microextraction
Pineapple
Pineapples
Plant Extracts - analysis
Plant Extracts - isolation & purification
pulp
Rendering
slurries
Solid Phase Microextraction
Volatile Organic Compounds - analysis
Volatile Organic Compounds - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Combining qualitative data from the chromatographic structure of 2-D gas chromatography with flame ionization detection (GC×GC-FID) and that from gas chromatography-mass spectrometry (GC/MS) should result in a more accurate assignment of the peak identities than the simple analysis by GC/MS, where coelution of analytes is unavoidable in highly complex samples (rendering spectra unsuitable for qualitative purposes) or for compounds in very low concentrations. Using data from GC×GC-FID combined with GC/MS can reveal coelutions that were not detected by mass spectra deconvolution software. In addition, some compounds can be identified according to the structure of the GC×GC-FID chromatogram. In this article, the volatile fractions of fresh and dehydrated pineapple pulp were evaluated. The extraction of the volatiles was performed by dynamic headspace extraction coupled to solid-phase microextraction (DHS-SPME), a technique appropriate for slurries or solid matrices. Extracted analytes were then analyzed by GC×GC-FID and GC/MS. The results obtained using both techniques were combined to improve compound identifications.
ISSN:1615-9306
1615-9314
1615-9314
DOI:10.1002/jssc.201000697