Tenascin C may regulate the recruitment of smooth muscle cells during coronary artery development

Tenascin C (TNC) is an extracellular glycoprotein that is thought to be involved in tissue remodeling during organogenesis and regeneration. Using avian embryonic hearts, we investigated the spatiotemporal expression patterns of TNC during the formation of the proximal coronary artery. Immunohistoch...

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Bibliographic Details
Published in:Differentiation (London) 2011-06, Vol.81 (5), p.299-306
Main Authors: Ando, Katsumi, Takahashi, Makiko, Yamagishi, Toshiyuki, Miyagawa-Tomita, Sachiko, Imanaka-Yoshida, Kyoko, Yoshida, Toshimichi, Nakajima, Yuji
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Avian heart
Cell Culture Techniques
Cell Differentiation - physiology
Chick Embryo
Coronary vessel development
Coronary Vessels - embryology
Coronary Vessels - metabolism
Heart - embryology
Immunohistochemistry - methods
Muscle Development
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - metabolism
Organogenesis
Pericardium - cytology
Pericardium - embryology
Pericardium - metabolism
Quail
Smooth muscle cell
Tenascin - biosynthesis
Tenascin - genetics
Tenascin - metabolism
Tenascin C
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Summary:Tenascin C (TNC) is an extracellular glycoprotein that is thought to be involved in tissue remodeling during organogenesis and regeneration. Using avian embryonic hearts, we investigated the spatiotemporal expression patterns of TNC during the formation of the proximal coronary artery. Immunohistochemistry showed that TNC was deposited around the developing coronary stem and that TNC colocalized with vascular smooth muscle α-actin. A quail-chick chimera, in which a quail proepicardial organ (PEO) had been transplanted, showed that quail tissue-derived cells contributed to the establishment of the endothelial and mural cells of the proximal coronary artery, and the quail tissue-derived mural cells displayed TNC. Proepicardial cells cultured in TNC showed the myofibroblast/smooth muscle cell phenotype and neutralizing anti-TNC antibody suppressed the expression of smooth muscle markers. These observations suggest that TNC plays a role in the mural smooth muscle development of the nascent proximal coronary artery.
ISSN:0301-4681
1432-0436
DOI:10.1016/j.diff.2011.03.002