Responsiveness of the human airway in vitro during deep inspiration and tidal oscillation

In healthy individuals, deep inspiration produces bronchodilation and reduced airway responsiveness, which may be a response of the airway wall to mechanical stretch. The aim of this study was to examine the in vitro response of isolated human airways to the dynamic mechanical stretch associated wit...

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Published in:Journal of applied physiology (1985) 2011-06, Vol.110 (6), p.1510-1518
Main Authors: NOBLE, Peter B, JONES, Robyn L, THAYA NEEDI, Elangovan, CAIRNCROSS, Alvenia, MITCHELL, Howard W, JAMES, Alan L, MCFAWN, Peter K
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Aged
Airway management
Airway Resistance - drug effects
Analysis of Variance
Biological and medical sciences
Bronchi - drug effects
Bronchi - physiology
Bronchial Provocation Tests
Bronchoconstriction - drug effects
Bronchoconstrictor Agents - pharmacology
Dose-Response Relationship, Drug
Female
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Inhalation
Lungs
Male
Mechanotransduction, Cellular
Middle Aged
Oscillometry
Pressure
Respiration
Tidal Volume
Time Factors
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cited_by cdi_FETCH-LOGICAL-c435t-32d72f9385ce33494365bd99d309620dbd7d66f7024cbc18b72d4081f73c9a273
cites cdi_FETCH-LOGICAL-c435t-32d72f9385ce33494365bd99d309620dbd7d66f7024cbc18b72d4081f73c9a273
container_end_page 1518
container_issue 6
container_start_page 1510
container_title Journal of applied physiology (1985)
container_volume 110
creator NOBLE, Peter B
JONES, Robyn L
THAYA NEEDI, Elangovan
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MITCHELL, Howard W
JAMES, Alan L
MCFAWN, Peter K
description In healthy individuals, deep inspiration produces bronchodilation and reduced airway responsiveness, which may be a response of the airway wall to mechanical stretch. The aim of this study was to examine the in vitro response of isolated human airways to the dynamic mechanical stretch associated with normal breathing. Human bronchial segments (n = 6) were acquired from patients without airflow obstruction undergoing lung resection for pulmonary neoplasms. The side branches were ligated and the airways were mounted in an organ bath chamber. Airway narrowing to cumulative concentrations of acetylcholine (3 × 10(-6) M to 3 × 10(-3) M) was measured under static conditions and in the presence of "tidal" oscillations with intermittent "deep inspiration." Respiratory maneuvers were simulated by varying transmural pressure using a motor-controlled syringe pump (tidal 5 to 10 cmH(2)O at 0.25 Hz, deep inspiration 5 to 30 cmH(2)O). Airway narrowing was determined from decreases in lumen volume. Tidal oscillation had no effect on airway responses to acetylcholine which was similar to those under static conditions. Deep inspiration in tidally oscillating, acetylcholine-contracted airways produced potent, transient (
doi_str_mv 10.1152/japplphysiol.01226.2010
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The aim of this study was to examine the in vitro response of isolated human airways to the dynamic mechanical stretch associated with normal breathing. Human bronchial segments (n = 6) were acquired from patients without airflow obstruction undergoing lung resection for pulmonary neoplasms. The side branches were ligated and the airways were mounted in an organ bath chamber. Airway narrowing to cumulative concentrations of acetylcholine (3 × 10(-6) M to 3 × 10(-3) M) was measured under static conditions and in the presence of "tidal" oscillations with intermittent "deep inspiration." Respiratory maneuvers were simulated by varying transmural pressure using a motor-controlled syringe pump (tidal 5 to 10 cmH(2)O at 0.25 Hz, deep inspiration 5 to 30 cmH(2)O). Airway narrowing was determined from decreases in lumen volume. Tidal oscillation had no effect on airway responses to acetylcholine which was similar to those under static conditions. Deep inspiration in tidally oscillating, acetylcholine-contracted airways produced potent, transient (&lt;1 min) bronchodilation, ranging from full reversal in airway narrowing at low acetylcholine concentrations to ∼50% reversal at the highest concentration. This resulted in a temporary reduction in maximal airway response (P &lt; 0.001), without a change in sensitivity to acetylcholine. 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Deep inspiration in tidally oscillating, acetylcholine-contracted airways produced potent, transient (&lt;1 min) bronchodilation, ranging from full reversal in airway narrowing at low acetylcholine concentrations to ∼50% reversal at the highest concentration. This resulted in a temporary reduction in maximal airway response (P &lt; 0.001), without a change in sensitivity to acetylcholine. Our findings are that the mechanical stretch of human airways produced by physiological transmural pressures generated during deep inspiration produces bronchodilation and a transient reduction in airway responsiveness, which can explain the beneficial effects of deep inspiration in bronchial provocation testing in vivo.</abstract><cop>Bethesda, MD</cop><pub>American Physiological Society</pub><pmid>21310892</pmid><doi>10.1152/japplphysiol.01226.2010</doi><tpages>9</tpages></addata></record>
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source American Physiological Society:Jisc Collections:American Physiological Society Journals ‘Read Publish & Join’ Agreement:2023-2024 (Reading list); American Physiological Society Free
subjects Acetylcholine - pharmacology
Aged
Airway management
Airway Resistance - drug effects
Analysis of Variance
Biological and medical sciences
Bronchi - drug effects
Bronchi - physiology
Bronchial Provocation Tests
Bronchoconstriction - drug effects
Bronchoconstrictor Agents - pharmacology
Dose-Response Relationship, Drug
Female
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Inhalation
Lungs
Male
Mechanotransduction, Cellular
Middle Aged
Oscillometry
Pressure
Respiration
Tidal Volume
Time Factors
title Responsiveness of the human airway in vitro during deep inspiration and tidal oscillation
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