Male-specific DNA in the dioecious species Atriplex garrettii (Chenopodiaceae)

The mechanism of sex determination in dioecious species of the genus Atriplex (Chenopodiaceae) has not been determined. This paper reports the discovery of a male-specific DNA fragment in the diploid dioecious species A. garrettii DNA samples extracted individually from ten male and ten female plant...

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Bibliographic Details
Published in:American journal of botany 1998-02, Vol.85 (2), p.162-167
Main Authors: Ruas, Claudete F., Fairbanks, Daniel J., Evans, R. Paul, Stutz, Howard C., Andersen, W. Ralph, Ruas, Paulo M.
Format: Article
Language:English
Subjects:
Atriplex
Botany
bulk segregant selection
Chenopodiaceae
Chromosomes
Deoxyribonucleic acid
dioecy
DNA
Gels
Genetics
Genetics and Molecular Biology
Male animals
Molecular biology
Nucleotide sequences
Plant genetics
Plant reproduction
Plants
RAPD (random amplified polymorphic DNA) analysis
repetitive DNA
Sex chromosomes
Sex determination
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Summary:The mechanism of sex determination in dioecious species of the genus Atriplex (Chenopodiaceae) has not been determined. This paper reports the discovery of a male-specific DNA fragment in the diploid dioecious species A. garrettii DNA samples extracted individually from ten male and ten female plants were bulked by sex. Random amplified polymorphic DNA (RAPD) fragments were generated in the two bulks in order to identify markers that were polymorphic between male and female plants A total of 158 decamer primers were tested A 2075 base-pair (bp) male-specific DNA fragment generated with the OPAF-14 primer was identified. The fragment was cloned and partially sequenced and 24-mer primers that exclusively amplified this fragment were constructed When 124 male plants, 126 female plants, and one hermaphroditic plant were tested individually, the male-specific 2075-bp DNA fragment was present in the hermaphrodite and all but one of the male plants, and was absent in all female plants A smaller DNA fragment (∼1800 bp) that was homologous to the 2075-bp fragment was amplified from the single male plant that lacked the 2075-bp fragment Cytogenetic analysis revealed no apparent heteromorphic sex chromosomes. These observations suggest that sex determination in A garrettii is genetic, with no evidence of heteromorphic sex chromosomes
ISSN:0002-9122
1537-2197
DOI:10.2307/2446304