A V-shaped cationic dye for nonlinear optical bioimaging

A symmetric cationic molecule with D-π-A(+)-π-D architecture was synthesized with high two-photon absorption cross-section (σ(2) ≈ 1140 GM). Application as a marker in fluorescence microscopy of living cells revealed its presence inside the cell staining vesicular shape organelles in the cytoplasm....

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (26), p.7374-7376
Main Authors: Maçôas, Ermelinda, Marcelo, Gema, Pinto, Sandra, Cañeque, Tatiana, Cuadro, Ana M, Vaquero, Juan J, Martinho, José M G
Format: Article
Language:English
Subjects:
Approximation
Cationic
Coloring Agents - chemical synthesis
Coloring Agents - chemistry
Cross sections
Dyes
Fluorescence
HEK293 Cells
Humans
Markers
Microscopy
Microscopy, Fluorescence - methods
Optical Phenomena
Organelles
Quinolizines - chemical synthesis
Quinolizines - chemistry
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Summary:A symmetric cationic molecule with D-π-A(+)-π-D architecture was synthesized with high two-photon absorption cross-section (σ(2) ≈ 1140 GM). Application as a marker in fluorescence microscopy of living cells revealed its presence inside the cell staining vesicular shape organelles in the cytoplasm. Fluorescence lifetime imaging microscopy shows that it is also able to penetrate within the nucleus.
ISSN:1359-7345
1364-548X
DOI:10.1039/c1cc12163d