Localization of sialidase-positive cells expressing Mac-1 and immunoglobulin in the mouse thymus

We have sought an endogenous membrane bound sialidase acting at neutral pH in immune system, because the removal of sialic acid from cell surfaces will affect the cell-cell interaction directly or indirectly. The levels of activity of unique membrane-bound sialidase at neutral pH and also soluble si...

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Bibliographic Details
Published in:Glycoconjugate journal 2003-01, Vol.20 (6), p.375-384
Main Authors: Kijimoto-Ochiai, Shigeko, Doi, Naoko, Matsukawa, Hiroko, Fujii, Miwako, Tomobe, Koji
Format: Article
Language:English
Subjects:
Animals
B-Lymphocytes - metabolism
Cell Communication
Cell Lineage
Cell Membrane - metabolism
Cells
Copper
Copper - chemistry
Enzyme Inhibitors - pharmacology
Hydrogen-Ion Concentration
Immune system
Immunoglobulins - metabolism
Immunohistochemistry
Localization
Lymph nodes
Lymphocytes T
Mac-1 antigen
Mac1 protein
Macrophage-1 Antigen - metabolism
Mice
Mice, Inbred C3H
N-Acetylneuraminic acid
N-Acetylneuraminic Acid - analogs & derivatives
N-Acetylneuraminic Acid - pharmacology
Neuraminidase - biosynthesis
Neuraminidase - metabolism
pH effects
Phosphates - metabolism
Proteins
Spleen
Spleen - metabolism
T-Lymphocytes - metabolism
Thymus
Thymus Gland - metabolism
Time Factors
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Summary:We have sought an endogenous membrane bound sialidase acting at neutral pH in immune system, because the removal of sialic acid from cell surfaces will affect the cell-cell interaction directly or indirectly. The levels of activity of unique membrane-bound sialidase at neutral pH and also soluble sialidase are high in the thymus but low in the spleen and lymph nodes. These are thought to be plasma membrane and cytosolic types based on the behavior of inhibition by Cu(2+) and 2-deoxy-2, 3-dehydro-N-acetylneuraminic acid. Newly synthesized 5-bromo-4-chloro-3-indolyl-N-acetylnueraminic acid was used for histochemical staining of sialidase-positive thymic cells, and the results showed positive cells sparsely distributed in the corticomedullar region or medullary region of the thymus. They expressed immunoglobulin and Mac-1 antigen on their surfaces. These cells must therefore be of a B cell lineage, not a T cell lineage. We also found that some vessels in the thymus were sialidase-positive.
ISSN:0282-0080
1573-4986
DOI:10.1023/b:glyc.0000033994.99464.ce