Macrophage surface glycoproteins binding to galectin-3 (Mac-2-antigen)

• Published in: Glycoconjugate journal 1997-02, Vol.14 (2), p.267-274
• Main Authors: Dong, S, Hughes, R C
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antigens, CD - metabolism; Antigens, Differentiation - metabolism; Carrier Proteins - metabolism; Cells; Chromatography, Affinity; Female; Fusion Regulatory Protein-1; Galectin 3; Lysosomal Membrane Proteins; Macrophage-1 Antigen - metabolism; Macrophages - chemistry; Macrophages - metabolism; Macrophages, Peritoneal - chemistry; Macrophages, Peritoneal - metabolism; Medical research; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - immunology; Membrane Glycoproteins - metabolism; Mice; Mice, Inbred BALB C; Precipitin Tests; Proteins; Rats; Sequence Analysis
• ISSN: 0282-0080; 1573-4986
• DOI: 10.1023/A:1018554124545

Galectin-3 (formerly called Mac-2 antigen) is a approximately 30 kDa carbohydrate-binding protein expressed on the surface of inflammatory macrophages and several macrophage cell lines. We have purified from lysates of the murine macrophage cell line WEHI-3 glycoproteins that bind to a galectin-3 affinity column. Several of these receptors are labelled after biotinylation of intact cells showing their location at the cell surface. N-terminal aminoacid sequencing of intact galectin-3-binding glycoproteins isolated from preparative SDS-gels or of chemically derived fragments showed several homologies with known proteins and identification was confirmed by immunoprecipitation with specific antibodies. The glycoproteins were shown to be: the alpha-subunit(CD11b) of the CD11b/CD18 integrin(Mac-1 antigen); the lysosomal membrane glycoproteins LAMPs 1 and 2 which are known in part to be expressed at cell surfaces; the Mac-3 antigen, a mouse macrophage differentiation antigen defined by the M3/84 monoclonal antibody and related immunochemically to LAMP-2; the heavy chain of CD98, a 125 kDa heterodimeric glycoprotein identified by the 4F2/RL388 monoclonal antibodies respectively on human and mouse monocytes/macrophages and on activated T cells. Further studies showed that CD11b/CD18, CD98 and Mac-3 are major surface receptors for galectin-3 on murine peritoneal macrophages elicited by thioglycollate.