Role of the Y-Family DNA Polymerases YqjH and YqjW in Protecting Sporulating Bacillussubtilis Cells from DNA Damage

The role played by the Y-family DNA polymerases YqjH and YqjW in protecting sporulating cells of Bacillussubtilis from DNA damage was determined. The absence of either yqjH and/or yqjW not only reduced sporulation efficiency but also sensitized the sporulating cells to hydrogen peroxide, tert-butylh...

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Bibliographic Details
Published in:Current microbiology 2010-04, Vol.60 (4), p.263-267
Main Authors: Rivas-Castillo, Andrea M, Yasbin, Ronald E, Robleto, E, Nicholson, Wayne L, Pedraza-Reyes, Mario
Format: Article
Language:English
Subjects:
DNA damage
DNA-directed DNA polymerase
Hydrogen peroxide
Mitomycin C
Mutation
Radiation
Sporulation
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Summary:The role played by the Y-family DNA polymerases YqjH and YqjW in protecting sporulating cells of Bacillussubtilis from DNA damage was determined. The absence of either yqjH and/or yqjW not only reduced sporulation efficiency but also sensitized the sporulating cells to hydrogen peroxide, tert-butylhydroperoxide (t-BHP), mitomycin-C (M-C), and UV-C radiation. Moreover, these DNA-damaging agents increased the mutation frequency of wild-type sporulating cells to 4-azaleucine, but the production of mutants was YqjH- and YqjW-dependent. In conclusion, the results presented here indicate that YqjH/YqjW-dependent-translesion synthesis (TLS) operates in sporulating B.subtilis cells and contributes in processing spontaneous and artificially induced genetic damage, which is apparently required for an efficient sporulation process.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-009-9535-3