Evaluation of antioxidant properties of medical plants using microbial test systems

The antioxidant activities of extracts from leaves of the medicinal plants growing in Siberia were examined. Total antioxidant activity was determined using in vitro methods including DPPH (2,2-diphenyl-1-picrylhydrazyl radical) free radical scavenging assay, chelating capacity assay with ferrozine,...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2010-12, Vol.26 (12), p.2269-2276
Main Authors: Smirnova, Galina V., Vysochina, Galina I., Muzyka, Nadezda G., Samoylova, Zoya Y., Kukushkina, Tatyana A., Oktyabrsky, Oleg N.
Format: Article
Language:English
Subjects:
Antioxidants
Applied Microbiology
Bacteria
Biochemistry
Biomedical and Life Sciences
Biotechnology
Chelation
E coli
Environmental Engineering/Biotechnology
Escherichia coli
Ethanol
Filipendula vulgaris
Free radicals
Herbal medicine
Hydrogen peroxide
Laboratories
Life Sciences
Medicinal plants
Microbiology
Microorganisms
Natural products
Original Paper
Plant extracts
Polyphenols
Pyrola rotundifolia
Studies
Test systems
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Summary:The antioxidant activities of extracts from leaves of the medicinal plants growing in Siberia were examined. Total antioxidant activity was determined using in vitro methods including DPPH (2,2-diphenyl-1-picrylhydrazyl radical) free radical scavenging assay, chelating capacity assay with ferrozine, evaluation of capacity to protect plasmid DNA against oxidative damage, measurement of H 2 O 2 production, and measurement of total flavonoid and tannin content as well. Using in vivo experiments, we also evaluated capacities of the plant extracts to protect bacteria Escherichia coli against bacteriostatic and bactericidal effects of H 2 O 2 , and influence of the plant extracts on expression of antioxidant gene katG , encoding catalase. The extracts from Chamerion angustifolium , Filipendula vulgaris and Pyrola rotundifolia indicated the highest levels of antioxidant activity both in vivo and in vitro. Our data suggest that the extracts of the tested plants may provide antioxidant effects on bacteria simultaneously through different pathways, including direct radical scavenging, iron chelation and induction of genes encoding antioxidant enzymes.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-010-0417-4