Amplification of repetitive DNA and origin of a rare chromosomal sex bivalent in Deltochilum (Calhyboma) verruciferum (Coleoptera, Scarabaeidae)

The most intriguing karyotypic variation in the Coleoptera involves variation in sex chromosome structure, origin and behavior. In this report we describe chromosomal characteristics of Deltochilum (Calhyboma) verruciferum (Coleoptera, Scarabaeidae) using conventional and differential cytogenetic te...

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Bibliographic Details
Published in:Genetica 2010-02, Vol.138 (2), p.191-195
Main Authors: Cabral-de-Mello, Diogo Cavalcanti, de Cássia de Moura, Rita, de Souza, Maria José
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Biomedical and Life Sciences
Chromosomes
Coleoptera
Coleoptera - cytology
Coleoptera - genetics
Coleoptera - metabolism
Deoxyribonucleic acid
Differentiation
DNA
Evolutionary Biology
Heterochromatin - metabolism
Human Genetics
Karyotypes
Life Sciences
Male
Meiosis - genetics
Microbial Genetics and Genomics
Nucleic Acid Amplification Techniques
Plant Genetics and Genomics
Repetitive Sequences, Nucleic Acid - genetics
Sex Chromosome Aberrations
Sex Chromosomes
Silver
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Summary:The most intriguing karyotypic variation in the Coleoptera involves variation in sex chromosome structure, origin and behavior. In this report we describe chromosomal characteristics of Deltochilum (Calhyboma) verruciferum (Coleoptera, Scarabaeidae) using conventional and differential cytogenetic techniques, with emphasis on the description of a rare sex bivalent in the Coleoptera and dispersion of repetitive DNA. This species shows 2n = 20,XYp with biarmed chromosomes. Analysis of constitutive heterochromatin through C-banding revealed mainly diphasic autosomes with short heterochromatic arms, while the X was completely heterochromatic and the Y was heterochromatic in the long arm. This pattern was confirmed through the use of CMA₃ fluorochrome that stained all heterochromatic regions. Silver nitrate staining marked all heterochromatic regions and the lumen of the sex bivalent in metaphase I. These results indicated that karyotypic differentiation in D. (C.) verruciferum involved autosomal amplification and dispersion of repetitive DNA and the origin of unusual sex chromosomes (XYp), generating a unique karyotype in this species.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-009-9409-4