Effect of cytokinins on oxidative stress in tobacco plants under nitrogen deficiency

• Published in: Environmental and experimental botany 2011-09, Vol.72 (2), p.167-173
• Main Authors: Rubio-Wilhelmi, M.M., Sanchez-Rodriguez, E., Rosales, M.A., Begoña, Blasco, Rios, J.J., Romero, L., Blumwald, E., Ruiz, J.M.
• Format: Article
• Language: English
• Subjects: Antioxidant system; Biological and medical sciences; Biomass; Cytokinins; Fundamental and applied biological sciences. Psychology; Nitrogen deficiency; Oxidative stress; Tobacco plants
• ISSN: 0098-8472; 1873-7307
• DOI: 10.1016/j.envexpbot.2011.03.005

► N-deficiency-induced senescence, the rise in ROS trigger oxidative stress. ► Transgenic plants expressing IPT gene coding the rate-limiting step in CKs synthesis. ► WT plants under N-limited, reduced biomass and increased the generation of ROS. ► Transgenic plants under N-limited, did not produce high ROS and maintained biomass. ► The expression of P SARK∷IPT in plants could improve nitrogen use efficiency. Wild type and transgenic tobacco plants expressing isopentenyltransferase, a gene coding the rate-limiting step in cytokinin synthesis, were grown under limited nitrogen (N) conditions. Our results indicated that the WT plants subjected to N deficiency displayed reduced biomass and relative growth rates, increased levels of oxidative damage and reduced foliar concentrations of the different N forms. However, the transgenic plants expressing P SARK∷IPT, in spite of showing a significant decline in all the N forms in the leaf, avoided the alteration of the oxidative metabolism and maintained biomass and the relative growth rates at control levels, under suboptimal N conditions. These results suggest that the increased cytokinin synthesis in the transgenic plants is an effective mechanism to improve N-use efficiency.