Assessment of Cadmium Genotoxicity in Peripheral Blood and Bone Marrow Tissues of Male Wistar Rats

In this study, Cadmium (Cd) genotoxicity was investigated in both bone marrow and peripheral blood treatment using rat micronucleus technique as genotoxicity test at acute and chronic treatment in the same animals. This study evaluated the frequency of micronuclei in the peripheral blood and bone ma...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology mechanisms and methods 2009-02, Vol.19 (2), p.135-140
Main Authors: Çelik, Ayla, Büyükakilli, Belgin, Çimen, Burak, Ta delen, Bahar, Öztürk, M. kbal, Eke, Dilek
Format: Article
Language:English
Subjects:
Animals
Bone Marrow - drug effects
Cadmium
Cadmium Chloride - toxicity
DNA - drug effects
Erythrocytes - cytology
Erythrocytes - drug effects
Female
Genotoxicity
Humans
Male
Micronucleus
Micronucleus Tests
Mitomycin - pharmacology
Nucleic Acid Synthesis Inhibitors - pharmacology
Polychromatic Erythrocytes
Rats
Rats, Wistar
Toxicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, Cadmium (Cd) genotoxicity was investigated in both bone marrow and peripheral blood treatment using rat micronucleus technique as genotoxicity test at acute and chronic treatment in the same animals. This study evaluated the frequency of micronuclei in the peripheral blood and bone marrow of male rats treated with unique cadmium dose (15 mg/kg. body w/day) by gavage for 60 days and acute treatment for 24 h, respectively. Mitomycin C (MMC) 2 mg/kg body wt was used as a positive control. This study shows that cadmium chloride treatment significantly induced the frequency of micronucleus in polychromatic erythrocytes in both tibia bone marrow and peripheral blood (p < 0.001, p < 0.01, respectively). This increase in micronucleus frequency shows that cadmium has a genotoxic effect on bone marrow and peripheral blood at this level. Also, in order to determine cytotoxicity in bone marrow and peripheral blood, the ratio of polychromatic erythrocytes to normochromatic erythrocytes was calculated in bone marrow and peripheral blood. Cd treatment decreased this ratio in only bone marrow. The results of this study demonstrate that Cd has both toxic and genotoxic potential in bone marrow and only genotoxic potential in peripheral blood. There is a significant difference between the control group and exposed group, including acute and chronic treatment for blood Cd level (p < 0.001). No significant difference was found between acute and chronic exposure group (p > 0.05).
ISSN:1537-6516
1537-6524
DOI:10.1080/15376510802354979