Measurement of methamphetamine on surfaces using surface plasmon resonance

• Published in: Toxicology mechanisms and methods 2009-09, Vol.19 (6-7), p.416-421
• Main Authors: Smith, Jerome P., Martin, Aaron, Sammons, Deborah L., Striley, Cynthia, Biagini, Raymond, Quinn, John, Cope, Rick, Snawder, John E.
• Format: Article
• Language: English
• Subjects: Biological Assay - methods; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Competitive immunoassay; Equipment Design; Methamphetamine - analysis; methamphetamine surface measurement; Reproducibility of Results; Sensitivity and Specificity; surface plasmon resonance (SPR); Surface Plasmon Resonance - instrumentation; Surface Plasmon Resonance - methods; Surface Properties
• ISSN: 1537-6516; 1537-6524
• DOI: 10.1080/15376510903114959

Field methods are needed to assess the contamination of surfaces by methamphetamine from illicit drug manufacturing. This study performed a feasibility study on the use of a surface plasmon resonance (SPR) based instrument (SensiQ Discovery) in the evaluation of surface contamination by methamphetamine. The main goal was to see if the method could be sensitive enough for field measurements. A competitive immunochemical assay was developed for the instrument which was able to measure methamphetamine at 9 ng/ml with a range of 9-250 ng/ml. Methamphetamine was spiked onto ceramic tiles and the assay was able to detect methamphetamine contamination at 25 ng/100 cm2, which is below the 50 ng/100 cm2 standard used for surface cleanup assessment. The instrument is compact and mobile and is sensitive enough for use for measurement of methamphetamine on surfaces, so it is a candidate for a field method for methamphetamine surface contamination. Its use for this application will require further development of the instrument to make it more convenient to use. Also further evaluation of ruggedness and use of the instrument under various environmental conditions such as temperature and humidity are needed to define conditions under which the instrument can be employed in field measurements.