Effects of extracellular calcium concentration on neurite outgrowth in PC12 cells by staurosporine

► Staurosporine induces neurite outgrowth in PC12. ► Increasing extracellular Ca2+ increases staurosporine-induced neurite outgrowth in PC12. ► staurosporine uses the extracellular calcium ions to affect neurite outgrowth in PC12. Staurosporine as an inhibitor of protein kinases can induce neuronal...

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Bibliographic Details
Published in:Neuroscience letters 2011-07, Vol.498 (1), p.1-5
Main Authors: Zhaleh, Hossein, Azadbakht, Mehri, Pour, Ali Bidmeshki
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Axonogenesis
Biological and medical sciences
Calcium (extracellular)
Calcium - metabolism
Cell Differentiation - drug effects
Cytotoxicity
Data processing
Differentiation
Enzyme Inhibitors - pharmacology
Extracellular Ca2
Extracellular Fluid - chemistry
Extracellular Fluid - metabolism
Fundamental and applied biological sciences. Psychology
Ions
Nervous system
Neurite outgrowth
Neurites - drug effects
Neurites - metabolism
Neurons - cytology
Neurons - drug effects
Neurons - metabolism
PC12 Cells
Pheochromocytoma cells
Protein kinase C
Rats
Staurosporine
Staurosporine - pharmacology
Vertebrates: nervous system and sense organs
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Summary:► Staurosporine induces neurite outgrowth in PC12. ► Increasing extracellular Ca2+ increases staurosporine-induced neurite outgrowth in PC12. ► staurosporine uses the extracellular calcium ions to affect neurite outgrowth in PC12. Staurosporine as an inhibitor of protein kinases can induce neuronal differentiation in PC12 cells. We investigated the role of extracellular Ca2+ on staurosporine inducing neurite outgrowth in PC12 cells. The cells were cultured during cell differentiation in the presence of 214nM staurosporine with 0.0–0.7 Ca2+mM as treatment media. We obtained the fraction of neurite-bearing cells, total neurite length and the percentage of cytotoxiciy. The results showed that decrease or increase of extracellular calcium ions resulted in correspondingly significant decrease or increase in total neurite length and cell differentiation in treated cells. With an increase of extracellular calcium concentration from 0.0 to 0.7mM, the percentage of cytotoxicity of the PC12 cells decreased (p
ISSN:0304-3940
1872-7972
DOI:10.1016/j.neulet.2011.04.020