Biocompatible multi-address 3D cell assembly in microfluidic devices using spatially programmable gel formation

Programmable 3D cell assembly under physiological pH conditions is achieved using electrodeposited stimuli-responsive alginate gels in a microfluidic device, with parallel sidewall electrodes enabling direct observation of the cell assembly. Electrically triggered assembly and subsequent viability o...

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Bibliographic Details
Published in:Lab on a chip 2011-07, Vol.11 (14), p.2316-2318
Main Authors: Cheng, Yi, Luo, Xiaolong, Tsao, Chen-Yu, Wu, Hsuan-Chen, Betz, Jordan, Payne, Gregory F, Bentley, William E, Rubloff, Gary W
Format: Article
Language:English
Subjects:
Alginates - chemistry
Animals
Cell Line
Electrochemical Techniques
Electrodes
Escherichia coli - genetics
Escherichia coli - metabolism
Gels - chemistry
Glucuronic Acid - chemistry
Hexuronic Acids - chemistry
Hydrogen-Ion Concentration
Luminescent Proteins - metabolism
Mice
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
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Summary:Programmable 3D cell assembly under physiological pH conditions is achieved using electrodeposited stimuli-responsive alginate gels in a microfluidic device, with parallel sidewall electrodes enabling direct observation of the cell assembly. Electrically triggered assembly and subsequent viability of mammalian cells is demonstrated, along with spatially programmable, multi-address assembly of different strains of E. coli cells. Our approach enables in vitro study of dynamic cellular and inter-cellular processes, from cell growth and stimulus/response to inter-colony and inter-species signaling.
ISSN:1473-0197
1473-0189
DOI:10.1039/c1lc20306a