A hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry

• Published in: Biomedical chromatography 2011-08, Vol.25 (8), p.908-912
• Main Authors: Zhao, Ming, Ma, Yan, Pan, Ying-Hong, Zhang, Chun-Hua, Yuan, Wen-Xia
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; antifungal protein; Antimicrobial Cationic Peptides - chemistry; Antimicrobial Cationic Peptides - isolation & purification; Antimicrobial Cationic Peptides - physiology; Chitinases - chemistry; Chitinases - isolation & purification; Chitinases - pharmacology; Chromatography, Ion Exchange; Drugs, Chinese Herbal; Electrophoresis, Polyacrylamide Gel; fast protein liquid chromatography; matrix-assisted laser desorption ionization time-of-flight mass spectrometry; Mitosporic Fungi - drug effects; Molecular Sequence Data; Molecular Weight; Morus - chemistry; Morus - enzymology; Peptide Mapping; peptide mapping fingerprint analysis; Plant Leaves - chemistry; Plant Lectins - chemistry; Plant Lectins - isolation & purification; Plant Lectins - physiology; Plant Proteins - chemistry; Plant Proteins - isolation & purification; Plant Proteins - pharmacology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.1543

Paper mulberry (Broussonetia papyrifera, syn. Morus papyrifera L.) is a Chinese traditional medicine and its low‐molecular‐weight extracts are reported to have antifungal activity. In this study, two proteins (PMAPI and PMAPII) with activity against Trichoderma viride were obtained from paper mulberry leaves with a fast protein liquid chromatography (FPLC) unit. The purification protocol employed (NH4)2SO4 precipitation, ion‐exchange chromatography and hydrophobic‐interaction chromatography on FPLC. Molecular masses were 18,798 Da for PMAPI, and 31,178 Da for PMAPII determined by Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry. Peptide mapping fingerprint analysis showed that PMAPI has no peptides similar to PMAPII. N‐terminal amino acid sequencing revealed that PMAPI is a hevein‐like protein, and PMAPII is a class I chitinase. They both had a half‐maximal inhibitory concentration (IC50) of 0.1 µg/µL against T. viride. This is the first report of high‐molecular‐weight extracts with antifungal activity from paper mulberry. Copyright © 2011 John Wiley & Sons, Ltd.