Development of a molecularly imprinted polymer for selective extraction followed by liquid chromatographic determination of sitagliptin in rat plasma and urine

• Published in: Talanta (Oxford) 2011-08, Vol.85 (2), p.950-957
• Main Authors: Rao, R. Nageswara, Maurya, Pawan K., Khalid, Sara
• Format: Article
• Language: English
• Subjects: Adsorption; Analytical chemistry; Animals; Blood Chemical Analysis - methods; Calibration; Chemical Fractionation - methods; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Exact sciences and technology; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Kinetics; Limit of Detection; Linear Models; Molecular Imprinting - methods; Molecularly imprinted polymer; Other chromatographic methods; Polymers - chemical synthesis; Pyrazines - analysis; Pyrazines - blood; Pyrazines - isolation & purification; Pyrazines - urine; Rat plasma; Rats; Reproducibility of Results; Sitagliptin; Sitagliptin Phosphate; Solid-phase extraction; Solvents - chemistry; Triazoles - analysis; Triazoles - blood; Triazoles - isolation & purification; Triazoles - urine; Urinalysis - methods; Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2011.05.002

A novel water-compatible molecularly imprinted solid-phase extraction (MISPE) combined with zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) method for selective extraction and determination of sitagliptin in rat serum and urine was developed and validated. The effects of progenic solvents, pH, cross linker and amount of monomer were studied to optimize the efficiency and selectivity. The adsorption kinetics and isotherms were measured. The molecularly imprinted polymer (MIP) showed good specific adsorption capacity with an optimum of 180 mg/g at pH 7.5 and selective extraction of sitagliptin from rat plasma and urine. The recovery of sitagliptin from rat urine and plasma was >98%. The limits of detection (LOD) and quantification (LOQ) were 0.03 and 0.10 μg/L respectively. The proposed method overcomes the matrix effects of phospholipids generally encountered while preparation of plasma samples by precipitation of proteins.