Identification of genes expressed during hair follicle induction

The hair follicle is one of the skin appendages that develops through reciprocal epithelial‐mesenchymal interactions. Although a large number of studies have been made on the mechanisms of hair follicle development, the whole molecular mechanism that governs hair follicle development remains poorly...

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Bibliographic Details
Published in:Journal of dermatology 2011-07, Vol.38 (7), p.674-679
Main Authors: KIYOZUMI, Daiji, OSADA, Aki, SUGIMOTO, Nagisa, WEBER, Charles N., ONO, Yuichi, IMAI, Toshio, OKADA, Akiko, SEKIGUCHI, Kiyotoshi
Format: Article
Language:English
Subjects:
Animals
Appendages
Base Sequence
cDNA subtraction
Development
DNA Primers - genetics
DNA, Complementary - genetics
epithelial-mesenchymal interaction
Epithelial-Mesenchymal Transition - genetics
Female
Follicles
Gene Expression Regulation, Developmental
Hair
hair follicle
Hair Follicle - growth & development
Hair Follicle - metabolism
In Situ Hybridization
Mice
Mice, Inbred ICR
Molecular modelling
Morphogenesis
Morphogenesis - genetics
Polymerase chain reaction
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
Skin
Vibrissae - growth & development
Vibrissae - metabolism
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Summary:The hair follicle is one of the skin appendages that develops through reciprocal epithelial‐mesenchymal interactions. Although a large number of studies have been made on the mechanisms of hair follicle development, the whole molecular mechanism that governs hair follicle development remains poorly defined. To further understand the molecular basis of hair follicle development, it is necessary to identify genes that drive hair morphogenesis. As an initial approach, we attempted to identify gene products associated with mouse hair follicle development. Genes upregulated in the vibrissal hair placodes were screened by polymerase chain reaction (PCR)‐based cDNA subtraction. The genes thus isolated were evaluated for their hair development‐associated induction and spatiotemporal expression by quantitative reverse‐transcription‐PCR analysis and whole‐mount in situ hybridization, respectively. Finally, we identified four genes whose upregulation and spatiotemporal expression in developing hair follicles were confirmed. Successful identification of novel hair development‐associated genes will be informative as clues for further characterization of hair follicle development at the molecular level.
ISSN:0385-2407
1346-8138
DOI:10.1111/j.1346-8138.2010.01050.x