Quantitative Analysis of Thymidine Kinase 1 and 5'(3')-Deoxyribonucleotidase mRNA Expression: The Role of Fluorothymidine Uptake

Thymidine kinase 1 (TK1) and 5'(3')-deoxyribonucleotidase (dNT1) regulate the metabolism of thymidine and its analogs. However, the expression patterns and roles of these enzymes in fluorothymidine (FLT) uptake have not been systemically studied. TK1 and dNT1 mRNAs were determined by quant...

Full description

Saved in:
Bibliographic Details
Published in:Anticancer research 2011-06, Vol.31 (6), p.2135-2139
Main Authors: Lee, Seung Jin, Lee, Haeng Jung, Moon, Dae Hyuk
Format: Article
Language:English
Subjects:
5'-Nucleotidase - biosynthesis
5'-Nucleotidase - genetics
Biological and medical sciences
Cell Line, Tumor
Colonic Neoplasms - enzymology
Colonic Neoplasms - genetics
Colonic Neoplasms - metabolism
Dideoxynucleosides - pharmacokinetics
HCT116 Cells
HT29 Cells
Humans
Lung Neoplasms - enzymology
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Medical sciences
Pneumology
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Thymidine Kinase - biosynthesis
Thymidine Kinase - genetics
Tritium
Tumors
Tumors of the respiratory system and mediastinum
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Thymidine kinase 1 (TK1) and 5'(3')-deoxyribonucleotidase (dNT1) regulate the metabolism of thymidine and its analogs. However, the expression patterns and roles of these enzymes in fluorothymidine (FLT) uptake have not been systemically studied. TK1 and dNT1 mRNAs were determined by quantitative PCR in 20 asynchronously growing lung and colon cancer cell lines. [3H]FLT uptake and doubling time were measured. The TK1/GAPDH values varied from 3.09×10(-3)±6.62×10(-4) to 2.44×10(-2)±8.49×10(-4) and dNT1/GAPDH values from 5.84×10(-4)±4.88×10(-5) to 1.59×10(-2)±1.20×10(-3). The correlation coefficient of TK1/GAPDH versus dNT1/GAPDH was 0.669 (p
ISSN:0250-7005
1791-7530