Soyasapogenol B and trans-22-dehydrocam- pesterol from common vetch (Vicia sativa L.) root exudates stimulate broomrape seed germination

• Published in: Pest management science 2011-08, Vol.67 (8), p.1015-1022
• Main Authors: Evidente, Antonio, Cimmino, Alessio, Fernández-Aparicio, Mónica, Rubiales, Diego, Andolfi, Anna, Melck, Dominique
• Format: Article
• Language: English
• Subjects: 22-dehydrocampesterol; Agronomy; Biological and medical sciences; Cholestadienols - isolation & purification; Cholestadienols - pharmacology; Crops; Fundamental and applied biological sciences. Psychology; Germination; Germination - drug effects; Herbicides; Magnetic Resonance Spectroscopy; Molecular Conformation; Oleanolic Acid - analogs & derivatives; Oleanolic Acid - isolation & purification; Oleanolic Acid - pharmacology; Orobanche; Orobanche - drug effects; Parasitic plants; Parasitic plants. Weeds; parasitic weeds; Phelipanche; Phytopathology. Animal pests. Plant and forest protection; Phytosterols - isolation & purification; Phytosterols - pharmacology; Plant Exudates - chemistry; Plant Roots - chemistry; Saponins - isolation & purification; Saponins - pharmacology; seed germination; soyasapogenol B; Spectrometry, Mass, Electrospray Ionization; Stereoisomerism; Vicia sativa - chemistry; Vicia sativa root exudates; Weeds
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.2153

BACKGROUND: Orobanche and Phelipanche species (the broomrapes) are root parasitic plants, some of which represent serious weed problems causing severe yield losses on important crops. Control strategies have largely focused on agronomic practices, resistant crop varieties and herbicides, albeit with marginal success. An alternative control method is the induction of suicidal seed germination with natural substances isolated from root exudates of host and non‐host plants. RESULTS: Soyasapogenol B [olean‐12‐ene‐3,22,24‐triol(3β,4β,22β)] and trans‐22‐dehydrocampesterol [(ergosta‐5,22‐dien‐3‐ol, (3β,22E,24S)] were isolated from Vicia sativa root exudates. They were identified by comparing their spectroscopic and optical properties with those reported in the literature. Soyasapogenol B was very specific, stimulating the germination of O. minor seeds only, whereas trans‐22‐dehydrocampesterol stimulated P. aegyptiaca, O. crenata, O. foetida and O. minor. CONCLUSION: Soyasapogenol B and trans‐22‐deydrocampesterol were isolated for the first time from Vicia sativa root exudates, and their biological activity as stimulants of Orobanche or Phelipanche sp. seed germination was reported. Copyright © 2011 Society of Chemical Industry