MicroRNA-10b induces glioma cell invasion by modulating MMP-14 and uPAR expression via HOXD10

• Published in: Brain research 2011-05, Vol.1389, p.9-18
• Main Authors: Sun, Lihua, Yan, Wei, Wang, Yingyi, Sun, Guan, Luo, Hui, Zhang, Junxia, Wang, Xiefeng, You, Yongping, Yang, Zhengxiang, Liu, Ning
• Format: Article
• Language: English
• Subjects: Anti-oncogenes; Antisense oligonucleotides; Apoptosis; Biological and medical sciences; Blotting, Western; brain; Brain tumors; cell invasion; Cell Line, Tumor; Complementarity; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Glioma; Glioma - genetics; Glioma - metabolism; Glioma - pathology; Glioma cells; Homeodomain Proteins - biosynthesis; HOXD10; Humans; Invasion; Malignancy; Matrix Metalloproteinase 14 - biosynthesis; Medical sciences; MicroRNA; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; MMP-14; Neoplasm Invasiveness - genetics; Neurology; non-coding RNA; nucleotide sequences; oligonucleotides; Oncogenes; Receptors, Urokinase Plasminogen Activator - biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Signal transduction; Signal Transduction - physiology; Transcription Factors - biosynthesis; Translation; translation (genetics); tumor suppressor genes; Tumors; Tumors of the nervous system. Phacomatoses; uPAR
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2011.03.013

Abstract MicroRNAs are small endogenous noncoding RNAs, which modulate target gene expression by binding with target mRNA sequences in the 3′untranslated region (UTR) with an imperfect complementarity that inhibits the mRNA translation. Many microRNAs have been reported to function as tumor oncogenes or anti-oncogenes. Recently, more and more microRNAs have been reported to contribute to a tumor's invasive potential. Here, we show that microRNA-10b (miR-10b) was over-expressed in glioma samples and directly associated with the glioma's pathological grade and malignancy. We also found that miR-10b induced glioma cell invasion by modulating tumor invasion factors MMP-14 and uPAR expression via the direct target HOXD10. The miR-10b/HOXD10/MMP-14/uPAR signaling pathway might contribute to the invasion of glioma. Accordingly, glioma cells lost their invasive ability when treated with specific antisense oligonucleotides (miR-10b inhibitors), suggesting that miR-10b could be used as a new bio-target to cure glioma.