Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis

Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges ( Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2011-06, Vol.33 (6), p.1177-1184
Main Authors: Jacob, Tiago R., Laia, Marcelo L., Ferro, Jesus A., Ferro, Maria I. T.
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Applied Microbiology
Bacterial Proteins - genetics
Bacterial Proton-Translocating ATPases - genetics
Base Sequence
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
C (programming language)
Citrus sinensis
Citrus sinensis - microbiology
Crop diseases
DNA Gyrase - genetics
DNA Primers - genetics
DNA-Directed RNA Polymerases - genetics
Fruits
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Profiling - methods
Genes
Genes, Bacterial
Life Sciences
Microbiology
Oranges
Original Research Paper
Plant Diseases - genetics
Plant Diseases - microbiology
Plant pathology
Plant resistance
Reverse Transcriptase Polymerase Chain Reaction
RNA, Bacterial - genetics
Stability
Sweets
Virulence - genetics
Xanthomonas
Xanthomonas - genetics
Xanthomonas - pathogenicity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges ( Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD , rpoB , gyrA and gyrB . The use of at least three reference genes is essential for accurate data normalization in Xcc.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-011-0552-5