Interleukin-6 Upregulates Expression of KDR and Stimulates Proliferation of Human Cerebrovascular Smooth Muscle Cells

• Published in: Journal of cerebral blood flow and metabolism 2007-03, Vol.27 (3), p.510-520
• Main Authors: Yao, Jianhua S, Zhai, Wenwu, Fan, Yongfeng, Lawton, Michael T, Barbaro, Nicholas M, Young, William L, Yang, Guo-Yuan
• Format: Article
• Language: English
• Subjects: Adult; Aged; Arteriovenous Malformations - metabolism; Blotting, Western; Brain - blood supply; Brain - metabolism; Cell Proliferation; Cells, Cultured; Cerebrovascular Circulation; Enzyme-Linked Immunosorbent Assay; Humans; Immunohistochemistry; Infant; Interleukin-6 - metabolism; Male; Matrix Metalloproteinase 9 - metabolism; Middle Aged; Muscle, Smooth, Vascular - metabolism; Myocytes, Smooth Muscle - metabolism; Phosphorylation; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Up-Regulation; Vascular Endothelial Growth Factor Receptor-2 - metabolism
• ISSN: 0271-678X; 1559-7016
• DOI: 10.1038/sj.jcbfm.9600365

Interleukin-6 (IL-6) may play multiple roles in angiogenesis and vascular remodeling. Our previous study showed that a promoter polymorphism (174G>C) in IL-6 is associated with brain arteriovenous malformation hemorrhage; tissue expression is related to genotype. In this study, we investigated the effects of IL-6 on human cerebral smooth muscle cells (HCSMCs) and smooth muscle cells isolated from brain arteriovenous malformation surgical specimens (AVM SMCs) and surgical controls (control HCSMCs—from structurally normal temporal lobe taken during surgical treatment of epilepsy patients). We found that IL-6 (1.1±0.27 versus 0.37±0.04 pg/mL, n=5, P