Standardization of fluorescence excitation–emission-matrices in aquatic milieu

Fluorescence excitation–emission-matrices (EEM) are a useful tool for water quality monitoring. Recent publications show the potential of the method for real time drinking water control. However, in fluorescence measurements there is still a need for standardization to make data interpretation compa...

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Bibliographic Details
Published in:Talanta (Oxford) 2011-07, Vol.85 (1), p.650-656
Main Authors: Goletz, Charlotte, Wagner, Martin, Grübel, Anika, Schmidt, Wido, Korf, Nathalie, Werner, Peter
Format: Article
Language:English
Subjects:
Analysis methods
Analytical chemistry
Applied sciences
Assessments
Chemistry
Emission
Emission correction
Environmental Monitoring - methods
Exact sciences and technology
Excitation
Excitation correction
Fluorescence
Humic Substances - analysis
Inner-filter effect
Natural water pollution
Normalization
Pollution
Quality Control
Reference Standards
Spectrometric and optical methods
Spectrometry, Fluorescence - standards
Standardization
Tryptophan
Tryptophan - analysis
Water - analysis
Water quality
Water Supply - analysis
Water treatment and pollution
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Summary:Fluorescence excitation–emission-matrices (EEM) are a useful tool for water quality monitoring. Recent publications show the potential of the method for real time drinking water control. However, in fluorescence measurements there is still a need for standardization to make data interpretation comparable. In this work a standardization procedure based on excitation and emission correction as well as normalization and optional inner filter effect correction is presented. By measurements of humic acid and tryptophan standards with two different spectrometers (LS 50 and LS 55 by PerkinElmer) the procedure application leads to comparable fluorescence intensities with relative standard deviations (median) of 6.6–8.4% and 10.6–12.0%, respectively. These small differences are not avoidable even if all possible correction methods are implemented and constant measurement conditions are given. The used BAM kit for emission correction induced good agreement in peak shape not only for single wavelengths but also for the whole EEM. As a consequence it is necessary to use identical equipment and identical experimental conditions in order to apply this method in fields of water quality control if small changes of fluorescence intensities are relevant for data assessment.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2011.04.045