Inhibitors of phosphatidylinositol 3-kinase and mTOR but not Akt enhance replication of bovine ephemeral fever virus

Non-segmented, negative-sense RNA viruses (NNSVs) depend on Akt (protein kinase B) for efficient replication. Infection with bovine ephemeral fever virus (BEFV) increases Akt phosphorylation. This study examined the effect of inhibition of phosphatidylinositol 3-kinase (PI3K)–Akt signalling on BEFV...

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Bibliographic Details
Published in:The veterinary journal (1997) 2011, Vol.187 (1), p.119-123
Main Authors: Ji, Wen T., Wang, Ying C., Lin, Feng L., Liao, Ming H., Shih, Wen L., Liu, Hung J.
Format: Article
Language:English
Subjects:
Akt
Animals
Bovine ephemeral fever virus
Cattle
Ephemeral Fever - virology
Ephemeral Fever Virus, Bovine - physiology
Inhibitors
mTOR
Phosphatidylinositol 3-kinase
Phosphatidylinositol 3-Kinase - antagonists & inhibitors
Phosphorylation
Proto-Oncogene Proteins c-akt - antagonists & inhibitors
Proto-Oncogene Proteins c-akt - metabolism
Signal Transduction
Sirolimus
TOR Serine-Threonine Kinases - antagonists & inhibitors
Viral replication
Virus Replication - drug effects
Virus Replication - physiology
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Summary:Non-segmented, negative-sense RNA viruses (NNSVs) depend on Akt (protein kinase B) for efficient replication. Infection with bovine ephemeral fever virus (BEFV) increases Akt phosphorylation. This study examined the effect of inhibition of phosphatidylinositol 3-kinase (PI3K)–Akt signalling on BEFV replication, since PI3K is the major upstream regulator of Akt. Treatment of BEFV-infected cells with two specific PI3K inhibitors (wortmannin and LY294002) enhanced replication of BEFV when compared to the effects of Akt inhibitors III and IV. BEFV antagonised the effects of PI3K inhibitors on Akt dephosphorylation. Inhibition of mTOR by rapamycin also enhanced replication of BEFV. The results provide evidences that inhibition of PI3K and mTOR has positive effects on replication of BEFV.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2009.11.003