Effects of prenatal ethanol exposure on rat brain radial glia and neuroblast migration

• Published in: Experimental neurology 2011-06, Vol.229 (2), p.364-371
• Main Authors: Aronne, María Paula, Guadagnoli, Tamara, Fontanet, Paula, Evrard, Sergio Gustavo, Brusco, Alicia
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Biological and medical sciences; Brain - cytology; Brain - drug effects; Brain - physiology; Cell Movement - drug effects; Cortical dysplasia; Corticogenesis; Ethanol - pharmacology; Eye Proteins - metabolism; Female; Fetal alcohol syndrome; Fluorescent Antibody Technique; Homeodomain Proteins - metabolism; Immunohistochemistry; Injuries of the nervous system and the skull. Diseases due to physical agents; Intermediate Filament Proteins - metabolism; Medical sciences; Microtubule-Associated Proteins - metabolism; Nerve Tissue Proteins - metabolism; Nestin; Neuroglia - drug effects; Neuroglia - physiology; Neurology; Neuronal migration; Neurons - drug effects; Neurons - physiology; Neuropeptides - metabolism; Paired Box Transcription Factors - metabolism; Pax6; PAX6 Transcription Factor; Pregnancy; Prenatal ethanol exposure; Prenatal Exposure Delayed Effects; Rats; Rats, Wistar; Repressor Proteins - metabolism; Traumas. Diseases due to physical agents; Vimentin - metabolism
• ISSN: 0014-4886; 1090-2430
• DOI: 10.1016/j.expneurol.2011.03.002

Prenatal ethanol exposure (PEE) induces morphologic and functional alterations in the developing central nervous system. The orderly migration of neuroblasts is a key process in the development of a layered structure such as the cerebral cortex (CC). From initial stages of corticogenesis, the transcription factor Pax6 is intensely expressed in neuroepithelial and radial glia cells (RGCs) and is involved in continual regulation of cell surface properties responsible for both cellular identity and radial migration. In the present work, one month before mating, during pregnancy and lactation, a group of female Wistar rats were fed a liquid diet with 5.9% (w/w) ethanol (EtOH), rendering moderate blood EtOH concentrations. Maternal gestational weight progression and fetal CC thickness were measured. CC from E12-P3 rats were examined for expression of vimentin, nestin, S-100b, Pax6 and doublecortin using immunohistochemical assays. RGCs expressing vimentin, nestin, S-100b and Pax6 had abnormal morphologies. The migration distance through the CC and the number of doublecortin-ir neuroblasts in germinative zones were decreased. We found significant morphologic defects on RGCs, a marked delay in neuronal migration, decreased numbers of neuroblasts, and decreased numbers of Pax6-ir cells in the CC as a consequence of exposure to ethanol during development. These observations suggest a sequence of toxic events that contribute to cortical dysplasia in offspring exposed to EtOH during gestation. ►The number and migration distance of doublecortin-ir neuroblasts are reduced during moderate prenatal ethanol exposure ►Nestin, vimentin and S100b expression is reduced in radial glial cells from E12 to P3 under moderate ethanol exposure ►Pax6-ir cells are reduced in number during corticogenesis under moderate ethanol exposure