PVA and BSA stabilized silver nanoparticles based surfaceaenhanced plasmon resonance probes for protein detection

To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443nm) when they wer...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2011-07, Vol.85 (2), p.138-144
Main Authors: Ananth, ANimrodh, Daniel, SCGKiruba, Sironmani, TAnitha, Umapathi, S
Format: Article
Language:English
Subjects:
Brain
Cellular
Mathematical models
Nanoparticles
Plasmons
Polyvinyl alcohols
Silver
Toxicity
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Summary:To perform biosensing using nanoparticles in solution, silver particles were coated with bovine serum albumin (BSA) and polyvinyl alcohol (PVA) as control stabilizer. The plasmon resonance (420nm) of the silver nanoparticles in solution was shifted slightly to longer wavelength (443nm) when they were coated with BSA. The biointeractions of these engineered nanoparticles were studied using a mouse model. No significant changes in behavior or toxicity were observed. The nanoparticles were detected in all tissues including the brain. Antibody recognition was monitored via the change in light absorption which accompanied binding, indicating that the particles can be used as a biosensor to gain more insight into cellular mechanisms governing the function of organs in general, and the blood brain barrier (BBB) and brain in particular.
ISSN:0927-7765
DOI:10.1016/j.colsurfb.2011.02.012