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Molecular cloning, characterization and expression analysis of the complement component C6 gene in grass carp

The complement system, as a representative of innate immunity, plays a key role in the host defense against infections. C6 is the member of complement components creating the membrane attack complex (MAC). In this study, we cloned and characterized the grass carp complement component C6 (gcC6) gene....

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Published in:Veterinary immunology and immunopathology 2011-05, Vol.141 (1), p.139-143
Main Authors: Shen, Yu-Bang, Zhang, Jun-Bin, Xu, Xiao-Yan, Li, Jia-Le
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Language:English
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container_title Veterinary immunology and immunopathology
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creator Shen, Yu-Bang
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description The complement system, as a representative of innate immunity, plays a key role in the host defense against infections. C6 is the member of complement components creating the membrane attack complex (MAC). In this study, we cloned and characterized the grass carp complement component C6 (gcC6) gene. Our data showed that gcC6 gene contained a 2724 bp open reading frame (ORF), a 237 bp 5′-untranslated region (UTR) and a 219 bp 3′-UTR. The deduced amino acid sequence of gcC6 showed 77.6% and 58.9% identity to zebrafish C6 and rainbow trout C6, respectively. GcC6 gene was expressed in a wide range of grass carp tissues, and the highest expression level of gcC6 was detected in the spleen and liver. Upon challenge with Aeromonas hydrophila, its expression was significantly up-regulated in muscle, trunk kidney, liver, head kidney, spleen, heart and intestine, whereas it was down-regulated in the brain and skin. The expression level of gcC6 was high at the unfertilized egg stage. It was significantly increased at 1 day post-hatching, but it was decreased at 10 days post-hatching. This result suggested that the complement C6 transcripts in early embryos were of maternal origin.
doi_str_mv 10.1016/j.vetimm.2011.02.001
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subjects Aeromonas hydrophila
Amino Acid Sequence
Animals
Carps - genetics
Carps - immunology
Cloning, Molecular
Complement C6 - genetics
Complement component C6
Ctenopharyngodon idella
Danio rerio
Freshwater
Gene Expression Profiling - veterinary
Genes - genetics
Genes - immunology
Grass carp
Inductive expression
Oncorhynchus mykiss
Open Reading Frames - genetics
Transcription
title Molecular cloning, characterization and expression analysis of the complement component C6 gene in grass carp
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