Preparations of intravenous immunoglobulins diminish the number and proinflammatory response of CD14+ CD16++ monocytes in common variable immunodeficiency (CVID) patients

• Published in: Clinical immunology (Orlando, Fla.) Fla.), 2011-05, Vol.139 (2), p.122-132
• Main Authors: Siedlar, Maciej, Strach, Magdalena, Bukowska-Strakova, Karolina, Lenart, Marzena, Szaflarska, Anna, Węglarczyk, Kazimierz, Rutkowska, Magdalena, Baj-Krzyworzeka, Monika, Pituch-Noworolska, Anna, Kowalczyk, Danuta, Grodzicki, Tomasz, Ziegler-Heitbrock, Loems, Zembala, Marek
• Format: Article
• Language: English
• Subjects: Adult; Allergy and Immunology; Antibodies, Monoclonal - pharmacology; Antigens, CD - metabolism; Biological and medical sciences; CD14 +CD16 ++ monocytes; CD32B; Common variable immunodeficiency; Common Variable Immunodeficiency - therapy; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; GPI-Linked Proteins - metabolism; HLA-DR Antigens - metabolism; Humans; Immunodeficiencies; Immunodeficiencies. Immunoglobulinopathies; Immunoglobulins, Intravenous - pharmacology; Immunoglobulins, Intravenous - therapeutic use; Immunopathology; Immunophenotyping; Interleukin-10 - metabolism; Interleukin-12 - metabolism; Intravenous immunoglobulins; Kinetics; Leukocyte Count; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - metabolism; Lipopolysaccharide Receptors - metabolism; Lipopolysaccharides - pharmacology; Male; Medical sciences; Middle Aged; Monocytes - cytology; Monocytes - drug effects; Monocytes - immunology; Monocytes - metabolism; Receptors, IgG - immunology; Receptors, IgG - metabolism; Tumor Necrosis Factor-alpha - metabolism; Young Adult
• ISSN: 1521-6616; 1521-7035
• DOI: 10.1016/j.clim.2011.01.002

Abstract We have studied the effect of intravenous immunoglobulins (IVIG) on monocyte subpopulations and cytokine production in patients with CVID. The absolute number of CD14+ CD16++ monocytes decreased on average 2.5-fold 4 h after IVIG and after 20 h returned to the baseline. The cytokine level in the supernatants of peripheral blood mononuclear cells (PBMC) after ex vivo LPS stimulation demonstrated the > 2-fold decrease in TNF production 4 h after IVIG. The TNF expression, which is higher in the CD14+ CD16++ monocytes, was decreased in these cells by IVIG in 4/7 CVID cases. In vitro exposure of the healthy individuals' monocytes to the IVIG preparation resulted in reduced TNF production, which was overcome by blockade of the FcγRIIB in the CD14+ CD16++ CD32Bhigh monocytes. Our data suggest that reduction in the number of CD14+ CD16++ monocytes and the blockade of their cytokine production via triggering CD32B can contribute to the anti-inflammatory action of IVIG.