The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus

The flavivirus NS5 protein is one of the most important proteins of the replication complex, and cellular proteins can interact with it. This study shows for the first time that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation....

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Bibliographic Details
Published in:Archives of virology 2011-06, Vol.156 (6), p.931-938
Main Authors: Bronzoni, Roberta V. M., Madrid, Maria C. F. S., Duarte, Danilo V. B., Pellegrini, Vanessa O. A., Pacca, Carolina C., Carmo, Ana C. V., Zanelli, Cleslei F., Valentini, Sandro R., Santacruz-Pérez, Carolina, Barbosa, João A. R. G., Lutz, Carol S., Rahal, Paula, Nogueira, Maurício L.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Animals
Binding Sites
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cercopithecus aethiops
Cloning
Conserved Sequence
Dengue fever
Encephalitis
Flavivirus
Fundamental and applied biological sciences. Psychology
HeLa Cells
Humans
Immunoprecipitation
Infectious Diseases
Medical Microbiology
Microbiology
Miscellaneous
Original Article
Pathogenesis
Plasmids
Polymerase Chain Reaction
Polypeptides
Protein Binding
Protein Interaction Domains and Motifs
Proteins
Ribonucleoprotein, U1 Small Nuclear - chemistry
Ribonucleoprotein, U1 Small Nuclear - metabolism
RNA polymerase
RNA, Viral
Two-Hybrid System Techniques
Vaccines
Vero Cells
Viral Nonstructural Proteins - chemistry
Viral Nonstructural Proteins - metabolism
Virology
Viruses
West Nile virus
Yellow fever virus
Yellow fever virus - genetics
Yellow fever virus - metabolism
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Summary:The flavivirus NS5 protein is one of the most important proteins of the replication complex, and cellular proteins can interact with it. This study shows for the first time that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation. We confirmed this interaction by GST-pulldown assay and by co-immunoprecipitation in YFV-infected cells. A region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A. This region was conserved among some flaviviruses of medical importance. The implications of this interaction for flavivirus replication are discussed.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-011-0927-x