Factors determining the spontaneous activation of splenic dendritic cells in culture

Dendritic cells (DCs) serve as a link between the innate and adaptive immune systems. The activation state of DCs is crucial in this role. However, when DCs are isolated from lymphoid tissues, purified and placed in culture they undergo ‘spontaneous’ activation. The basis of this was explored, using...

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Bibliographic Details
Published in:Innate immunity (London, England) England), 2011-06, Vol.17 (3), p.338-352
Main Authors: Vremec, David, O'Keeffe, Meredith, Wilson, Anne, Ferrero, Isabel, Koch, Ute, Radtke, Freddy, Scott, Bernadette, Hertzog, Paul, Villadangos, Jose, Shortman, Ken
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - genetics
Antigens, CD - metabolism
beta Catenin - genetics
Cadherins - metabolism
Cell Adhesion
Cell Communication - immunology
Cell Differentiation
Cells, Cultured
Chimera
Cytokines - genetics
Cytokines - metabolism
Dendritic Cells - immunology
Dendritic Cells - metabolism
Dendritic Cells - pathology
Histocompatibility Antigens Class II - genetics
Histocompatibility Antigens Class II - metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Spleen
Up-Regulation - immunology
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Summary:Dendritic cells (DCs) serve as a link between the innate and adaptive immune systems. The activation state of DCs is crucial in this role. However, when DCs are isolated from lymphoid tissues, purified and placed in culture they undergo ‘spontaneous’ activation. The basis of this was explored, using up-regulation of DC surface MHC II, CD40, CD80 and CD86 as indicators of DC activation. No evidence was found for DC damage during isolation or for microbial products causing the activation. The culture activation of spleen DCs differed from that of Langerhans cells when released from E-cadherin-mediated adhesions, since E-cadherin was not detected and activation still occurred with β-catenin null DCs. Much of the activation could be attributed to DC—DC interactions. Although increases in surface MHC II levels occurred under all culture conditions tested, the increase in expression of CD40, CD80 and CD86 was much less under culture conditions where such interactions were minimised. DC-to-DC contact under the artificial conditions of high DC concentration in culture induced the production of soluble factors and these, in turn, induced the up-regulation of co-stimulatory molecules on the DC surface.
ISSN:1753-4259
1753-4267
DOI:10.1177/1753425910371396