Vasoconstrictive neurovascular coupling during focal ischemic depolarizations

• Published in: Journal of cerebral blood flow and metabolism 2006-08, Vol.26 (8), p.1018-1030
• Main Authors: Shin, Hwa Kyoung, Dunn, Andrew K, Jones, Phillip B, Boas, David A, Moskowitz, Michael A, Ayata, Cenk
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blood. Blood coagulation. Reticuloendothelial system; Cerebellar Cortex - blood supply; Cerebellar Cortex - metabolism; Cerebellar Cortex - pathology; Cerebellar Cortex - physiopathology; Cerebrovascular Circulation - drug effects; Disease Models, Animal; Excitatory Amino Acid Agonists - pharmacology; Excitatory Amino Acid Antagonists - pharmacology; Infarction, Middle Cerebral Artery - drug therapy; Infarction, Middle Cerebral Artery - metabolism; Infarction, Middle Cerebral Artery - pathology; Infarction, Middle Cerebral Artery - physiopathology; Medical sciences; Mice; Neurology; Neuropharmacology; Neuroprotective agent; Neuroprotective Agents - pharmacology; Pharmacology. Drug treatments; Receptors, N-Methyl-D-Aspartate - agonists; Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors; Signal Transduction - drug effects; Vascular diseases and vascular malformations of the nervous system; Vasoconstriction - drug effects
• ISSN: 0271-678X; 1559-7016
• DOI: 10.1038/sj.jcbfm.9600252

Ischemic depolarizing events, such as repetitive spontaneous periinfarct spreading depolarizations (PIDs), expand the infarct size after experimental middle cerebral artery (MCA) occlusion. This worsening may result from increased metabolic demand, exacerbating the mismatch between cerebral blood flow (CBF) and metabolism. Here, we present data showing that anoxic depolarization (AD) and PIDs caused vasoconstriction and abruptly reduced CBF in the ischemic cortex in a distal MCA occlusion model in mice. This reduction in CBF during AD increased the area of cortex with 20% or less residual CBF by 140%. With each subsequent PID, this area expanded by an additional 19%. Drugs that are known to inhibit cortical spreading depression (CSD), such as N-methyl-D-aspartate receptor antagonists MK-801 and 7-chlorokynurenic acid, and σ-1 receptor agonists dextromethorphan and carbetapentane, did not reduce the frequency of PIDs, but did diminish the severity of episodic hypoperfusions, and prevented the expansion of severely hypoperfused cortex, thus improving CBF during 90 mins of acute focal ischemia. In contrast, AMPA receptor antagonist NBQX, which does not inhibit CSD, did not impact the deterioration in CBF. When measured 24 h after distal MCA occlusion, infarct size was reduced by MK-801, but not by NBQX. Our results suggest that AD and PIDs expand the CBF deficit, and by so doing negatively impact lesion development in ischemic mouse brain. Mitigating the vasoconstrictive neurovascular coupling during intense ischemic depolarizations may provide a novel hemodynamic mechanism of neuroprotection by inhibitors of CSD.