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Immunoglobulin M antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi in chronic chagasic patients
Abstract Previous works of our research group have demonstrated aspects of the humoral immune response of chronic Chagas disease using the cytoplasmatic repetitive antigen (CRA) and the flagellar repetitive antigen (FRA) of Trypanosoma cruzi . The aim of this work was to analyze the presence of spec...
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Published in: | Human immunology 2011-05, Vol.72 (5), p.402-405 |
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container_title | Human immunology |
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creator | Vasconcelos, Romero H.T Azevedo, Elisa A.N Cavalcanti, Maria G.A.M Silva, Edimilson D Ferreira, Antonio G.P Morais, Clarice N.L Gomes, Yara M |
description | Abstract Previous works of our research group have demonstrated aspects of the humoral immune response of chronic Chagas disease using the cytoplasmatic repetitive antigen (CRA) and the flagellar repetitive antigen (FRA) of Trypanosoma cruzi . The aim of this work was to analyze the presence of specific immunoglobulin M (IgM) antibodies in chronic chagasic patients using these recombinant antigens of T. cruzi . The positivity of IgM in chronic chagasic patients against CRA and FRA antigens was determined by indirect enzyme-linked immunosorbent assay. We reported no statistical significant differences between the levels of IgM for both recombinant antigens and the different chronic clinical forms of Chagas disease. However, a small proportion of chronic chagasic patients analyzed in this study was positive for this antibody isotype. The findings of this study indicate that the IgM antibodies cannot be used to elucidate the differences in the profile of humoral immune response among chronic chagasic patients with different clinical forms using the CRA and FRA recombinant antigens of T. cruzi. |
doi_str_mv | 10.1016/j.humimm.2011.02.015 |
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The aim of this work was to analyze the presence of specific immunoglobulin M (IgM) antibodies in chronic chagasic patients using these recombinant antigens of T. cruzi . The positivity of IgM in chronic chagasic patients against CRA and FRA antigens was determined by indirect enzyme-linked immunosorbent assay. We reported no statistical significant differences between the levels of IgM for both recombinant antigens and the different chronic clinical forms of Chagas disease. However, a small proportion of chronic chagasic patients analyzed in this study was positive for this antibody isotype. The findings of this study indicate that the IgM antibodies cannot be used to elucidate the differences in the profile of humoral immune response among chronic chagasic patients with different clinical forms using the CRA and FRA recombinant antigens of T. cruzi.</description><identifier>ISSN: 0198-8859</identifier><identifier>EISSN: 1879-1166</identifier><identifier>DOI: 10.1016/j.humimm.2011.02.015</identifier><identifier>PMID: 21371515</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Allergy and Immunology ; Antigens, Protozoan - genetics ; Antigens, Protozoan - immunology ; Antigens, Protozoan - metabolism ; Chagas Cardiomyopathy ; Chagas disease ; Chagas Disease - classification ; Chagas Disease - diagnosis ; Chagas Disease - immunology ; Chagas Disease - physiopathology ; Chronic Disease ; Disease Progression ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunoglobulin M ; Immunoglobulin M - blood ; Prognosis ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Protozoan Proteins - metabolism ; Recombinant antigens ; Recombinant Proteins - genetics ; Trypanosoma cruzi ; Trypanosoma cruzi - immunology ; Trypanosoma cruzi - pathogenicity</subject><ispartof>Human immunology, 2011-05, Vol.72 (5), p.402-405</ispartof><rights>American Society for Histocompatibility and Immunogenetics</rights><rights>2011 American Society for Histocompatibility and Immunogenetics</rights><rights>Copyright © 2011 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. 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The aim of this work was to analyze the presence of specific immunoglobulin M (IgM) antibodies in chronic chagasic patients using these recombinant antigens of T. cruzi . The positivity of IgM in chronic chagasic patients against CRA and FRA antigens was determined by indirect enzyme-linked immunosorbent assay. We reported no statistical significant differences between the levels of IgM for both recombinant antigens and the different chronic clinical forms of Chagas disease. However, a small proportion of chronic chagasic patients analyzed in this study was positive for this antibody isotype. The findings of this study indicate that the IgM antibodies cannot be used to elucidate the differences in the profile of humoral immune response among chronic chagasic patients with different clinical forms using the CRA and FRA recombinant antigens of T. cruzi.</description><subject>Allergy and Immunology</subject><subject>Antigens, Protozoan - genetics</subject><subject>Antigens, Protozoan - immunology</subject><subject>Antigens, Protozoan - metabolism</subject><subject>Chagas Cardiomyopathy</subject><subject>Chagas disease</subject><subject>Chagas Disease - classification</subject><subject>Chagas Disease - diagnosis</subject><subject>Chagas Disease - immunology</subject><subject>Chagas Disease - physiopathology</subject><subject>Chronic Disease</subject><subject>Disease Progression</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>Immunoglobulin M</subject><subject>Immunoglobulin M - blood</subject><subject>Prognosis</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Protozoan Proteins - metabolism</subject><subject>Recombinant antigens</subject><subject>Recombinant Proteins - genetics</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - immunology</subject><subject>Trypanosoma cruzi - pathogenicity</subject><issn>0198-8859</issn><issn>1879-1166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkk9v1DAQxS0EokvhGyCUG6cETxIn8QWpWlGo1KoSbc-W_2XrJbYXO6m0fHpmuy0HLj2N5fnNG-m9IeQj0AoodF-21f3infdVTQEqWlcU2CuygqHnJUDXvSYrCnwoh4HxE_Iu5y2ltKd9-5ac1ND0wICtyMOF90uImymqZXKhuCpkmJ2KxtlcyI10Ic_F-ucZfpviHGuyOnrlAmKP6MaGXMSxuE37nQwxRy8LnZY_rkA1fZ9icBorSmV87OTsbJjze_JmlFO2H57qKbk7_3a7_lFeXn-_WJ9dlrpth7nsgdaKtkpK1lM26kaOnA2cj1Ixq5SBBrgBa2vGDVdm6Lgxpu1r3Q3NyLB9Sj4fdXcp_l5snoV3WdtpksHGJYuh7-qWddC9THa4hDPOkGyPpE4x52RHsUvOy7QXQMUhGrEVx2jEIRpBa4HR4NinpwWL8tb8G3rOAoGvR8CiIQ_OJpE1mqWtcWj6LEx0L234X0BjpE7L6Zfd27yNSwpotgCRcUDcHM7jcB0AeBlt0zd_Ab4uuAE</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Vasconcelos, Romero H.T</creator><creator>Azevedo, Elisa A.N</creator><creator>Cavalcanti, Maria G.A.M</creator><creator>Silva, Edimilson D</creator><creator>Ferreira, Antonio G.P</creator><creator>Morais, Clarice N.L</creator><creator>Gomes, Yara M</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20110501</creationdate><title>Immunoglobulin M antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi in chronic chagasic patients</title><author>Vasconcelos, Romero H.T ; 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subjects | Allergy and Immunology Antigens, Protozoan - genetics Antigens, Protozoan - immunology Antigens, Protozoan - metabolism Chagas Cardiomyopathy Chagas disease Chagas Disease - classification Chagas Disease - diagnosis Chagas Disease - immunology Chagas Disease - physiopathology Chronic Disease Disease Progression Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin M Immunoglobulin M - blood Prognosis Protozoan Proteins - genetics Protozoan Proteins - immunology Protozoan Proteins - metabolism Recombinant antigens Recombinant Proteins - genetics Trypanosoma cruzi Trypanosoma cruzi - immunology Trypanosoma cruzi - pathogenicity |
title | Immunoglobulin M antibodies against CRA and FRA recombinant antigens of Trypanosoma cruzi in chronic chagasic patients |
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