Synthesis and characterization of a laminated hydroxyapatite/gelatin nanocomposite scaffold with controlled pore structure for bone tissue engineering

In this study, a nanostructured scaffold was designed for bone repair using hydroxyapatite (HA) and gelatin (GEL) as its main components. Nanopowders of HA were synthesized, and together with GEL, used to engineer a 3-dimensional nanocomposite combining 3 techniques of layer solvent casting, freeze-...

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Bibliographic Details
Published in:International journal of artificial organs 2010-02, Vol.33 (2), p.86-95
Main Authors: Azami, Mahmoud, Samadikuchaksaraei, Ali, Poursamar, Seyed Ali
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Bone (spongy)
Bone and Bones - anatomy & histology
Cell Culture Techniques - methods
Cell Line
Durapatite - chemistry
Gelatin - chemistry
Humans
Microscopy, Electron
Microscopy, Electron, Scanning
Nanocomposites - chemistry
Osteoblasts - cytology
Porosity
Solutions
Spectroscopy, Fourier Transform Infrared
Tissue Engineering - methods
Tissue Engineering - standards
Tissue Scaffolds - standards
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Summary:In this study, a nanostructured scaffold was designed for bone repair using hydroxyapatite (HA) and gelatin (GEL) as its main components. Nanopowders of HA were synthesized, and together with GEL, used to engineer a 3-dimensional nanocomposite combining 3 techniques of layer solvent casting, freeze-drying, and lamination. The results show that the scaffold possesses a 3-dimensional interconnected homogenous porous structure with a porosity of 82% and pore sizes ranging from 300 to 500 mum. It has also been shown that mechanical indices are in the range of spongy bones. Cultured osteoblast-like cells (SaOS-2) have shown an excellent level of cell attachment, migration, and penetration into the porosities of the nanocomposite scaffold. Here, we have shown that by a combination of widely available methods with simple experimental operations, nano-HA powders can be synthesized and used to make 3-dimensional HA/GEL nanocomposites in any desired shape, with mechanical properties comparable to spongy bone.
ISSN:0391-3988
1724-6040
DOI:10.1177/039139881003300204