Differentiation of AFS cells derived from the EGFP gene transgenic porcine fetuses

We have obtained the EGFP (enhanced green fluorescence protein) gene transgenic porcine fetuses before. The aims of this study were (i) to determine whether stem cells could be isolated from amniotic fluid of the transgenic porcine fetuses, and (ii) to determine if these stem cells could express EGF...

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Bibliographic Details
Published in:Cell biology international 2011-08, Vol.35 (8), p.835-839
Main Authors: Zheng, Yue‐Mao, Dang, Yong‐Hui, Xu, Yong‐Ping, Sai, Wu‐Jia‐Fu, An, Zhi‐Xing
Format: Article
Language:English
Subjects:
Amniotic Fluid - cytology
Amniotic Fluid - metabolism
amniotic fluid‐derived stem cell
Animals
Animals, Genetically Modified
Astrocytes - cytology
Astrocytes - metabolism
Cell Differentiation - physiology
Chondrocytes - cytology
Chondrocytes - metabolism
Collagen Type II - biosynthesis
differentiation
EGFP gene
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Fetus - cytology
Fetus - metabolism
Galactosylceramidase - biosynthesis
Green Fluorescent Proteins - biosynthesis
Green Fluorescent Proteins - genetics
Mitogen-Activated Protein Kinases - biosynthesis
Neurons - cytology
Neurons - metabolism
Octamer Transcription Factor-3 - biosynthesis
Osteoblasts - cytology
Osteoblasts - metabolism
Osteocalcin - biosynthesis
Osteonectin - biosynthesis
pig
Reverse Transcriptase Polymerase Chain Reaction
SOXB1 Transcription Factors - biosynthesis
Swine
Thy-1 Antigens - biosynthesis
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Summary:We have obtained the EGFP (enhanced green fluorescence protein) gene transgenic porcine fetuses before. The aims of this study were (i) to determine whether stem cells could be isolated from amniotic fluid of the transgenic porcine fetuses, and (ii) to determine if these stem cells could express EGFP and differentiate in vitro. The results demonstrated that stem cells could be isolated from amniotic fluid of the EGFP gene transgenic porcine fetuses and could express EGFP and differentiate in vitro. Undifferentiated AFSs (amniotic fluid‐derived stem cells) expressed POU5F1, THY1 and SOX2, while the following differentiation cells expressed markers for chondrogenic (COL2A1), osteogenic (osteocalcin and osteonectin) and neurogenic cells such as astrocyte (GFAP), oligodendrocyte (GALC) and neuron (NF, ENO2 and MAP).
ISSN:1065-6995
1095-8355
DOI:10.1042/CBI20100317