Down‐regulation of c‐Myc expression inhibits the invasion of bile duct carcinoma cells

Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c‐Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c‐Myc to drive unrestricted cell proliferation and to...

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Bibliographic Details
Published in:Cell biology international 2011-08, Vol.35 (8), p.799-802
Main Authors: Li, Zhuo‐Ri, Wu, Yi‐Fei, Ma, Chun‐Yang, Nie, Shen‐Dan, Mao, Xian‐Hai, Shi, Yong‐Zhong
Format: Article
Language:English
Subjects:
Bile Duct Neoplasms - genetics
Bile Duct Neoplasms - metabolism
Bile Duct Neoplasms - pathology
Bile Ducts, Intrahepatic - pathology
Cell Line, Tumor
Cell Proliferation - drug effects
cholangiocarcinoma
Cholangiocarcinoma - genetics
Cholangiocarcinoma - metabolism
Cholangiocarcinoma - pathology
Codon, Nonsense - genetics
Codon, Nonsense - pharmacology
c‐Myc
Down-Regulation - drug effects
Gene Expression Regulation, Neoplastic
Humans
invasion
Liver - pathology
Liver Neoplasms - metabolism
Neoplasm Invasiveness
Oligodeoxyribonucleotides, Antisense - genetics
Oligodeoxyribonucleotides, Antisense - pharmacology
Proto-Oncogene Proteins c-myc - biosynthesis
Proto-Oncogene Proteins c-myc - genetics
Transfection
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Summary:Cholangiocarcinoma is the second most common primary hepatic tumour originating from biliary tract epithelial cells with poor prognosis. Enhanced c‐Myc protein expression contributes to many aspects of tumour cell biology. Although the ability of c‐Myc to drive unrestricted cell proliferation and to inhibit cell differentiation had been well recognized, whether down‐regulated c‐Myc expression can inhibit tumour cell invasion still remains to be explored. The c‐Myc ASODN (antisense oligodeoxyribonucleotide) and NSODN (nonsense oligodeoxyribonucleotide) were designed, synthesized and transfected into human QBC939 bile duct carcinoma cells using the Lipofectamine 2000 reagent. The protein expression of c‐Myc was detected by Western blot. A transwell experiment was applied to evaluate the invasive capacity of the QBC939 cells. c‐Myc ASODN could significantly suppress the c‐Myc protein expression (P
ISSN:1065-6995
1095-8355
DOI:10.1042/CBI20110099