The effect of two point mutations in GDF-5 on ectopic bone formation in a b-tricalciumphosphate scaffold

The osteoinductivity of human growth-and-differentiation factor-5 (GDF-5) is well established, but a reduced amount of ectopic bone is formed compared to other members of the bone morphogenetic protein (BMP) family like BMP-2. We hypothesized that swap of two BMP-receptor-interacting residues of GDF...

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Bibliographic Details
Published in:Biomaterials 2010-05, Vol.31 (14), p.3878-3884
Main Authors: Kasten, Philip, Beyen, Ingo, Bormann, Dirk, Luginbuehl, Reto, Ploeger, Frank, Richter, Wiltrud
Format: Article
Language:English
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Summary:The osteoinductivity of human growth-and-differentiation factor-5 (GDF-5) is well established, but a reduced amount of ectopic bone is formed compared to other members of the bone morphogenetic protein (BMP) family like BMP-2. We hypothesized that swap of two BMP-receptor-interacting residues of GDF-5 to amino acids present in BMP-2 (methionine to valine at the sites 453 and 456) may improve the bone formation capacity of the mutant GDF-5. Heterotopic bone formation of a mutant GDF-5 coated b-TCP carrier was compared to carriers coated with similar amounts (10aaI14g) of GDF-5 and BMP-2 in SCID mice. Four week explants revealed 6-fold higher ALP activity in the mutant GDF-5 versus the wild type GDF-5 group (paa
ISSN:0142-9612
DOI:10.1016/j.biomaterials.2010.01.109