Effect of edelfosine on tumor and normal cells model membranes—A comparative study

This work is aimed at comparing the effect of edelfosine (Ed) on normal and tumor cell membrane imitated by the Langmuir monolayers prepared from cholesterol and phosphatidylcholines. To keep the differences in the fluidity of these membranes, model membrane of normal cell has been composed from sat...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2010-03, Vol.76 (1), p.366-369
Main Authors: Hąc-Wydro, Katarzyna, Dynarowicz-Łątka, Patrycja
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Cell Membrane - drug effects
Edelfosine (Ed)
Langmuir monolayers
Membrane Fluidity
Membranes, Artificial
Model cell membrane
Models, Biological
Phospholipid Ethers - pharmacology
Tumor cell membrane
Unilamellar Liposomes
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Summary:This work is aimed at comparing the effect of edelfosine (Ed) on normal and tumor cell membrane imitated by the Langmuir monolayers prepared from cholesterol and phosphatidylcholines. To keep the differences in the fluidity of these membranes, model membrane of normal cell has been composed from saturated phospholipid (DPPC) and contained a higher proportion of cholesterol (cholesterol:DPPC = 0.67) than tumor cell model membranes prepared from unsaturated phosphatidylcholine (cholesterol:POPC = 0.25). The results proved that the incorporation of edelfosine modifies the organization and interactions between molecules in both model systems. The interactions in cholesterol/DPPC/edelfosine monolayers are stronger than in cholesterol/DPPC film. On the other hand, the interactions in cholesterol/POPC/edelfosine system are weaker than in cholesterol/POPC monolayer, thus the incorporation of edelfosine is, from thermodynamical point of view, unfavorable for binary cholesterol/POPC monolayer. Edelfosine has been found to increase the fluidity of model membranes, however, at a lower concentration (up to 5% of edelfosine in model system) this compound affects only the condensation of tumor cell model membrane, while practically does not modify the organization of normal model system. It has been suggested that edelfosine inserts into tumor cellular membranes more easily than into normal cell membrane and cholesterol/edelfosine interactions determine the interactions between molecules in the investigated mixed monolayers.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2009.10.012