Direct electrochemistry of horseradish peroxidase immobilized on a monolayer modified nanowire array electrode

Vertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H 2O 2 biosensors were prepared by covalent immobili...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2010-02, Vol.25 (6), p.1313-1318
Main Authors: Xu, Ju, Shang, Fengjun, Luong, John H.T., Razeeb, Kafil M., Glennon, Jeremy D.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biotechnology
Direct electrochemistry
Electrochemistry - instrumentation
Enzymes, Immobilized - chemistry
Equipment Design
Equipment Failure Analysis
Fundamental and applied biological sciences. Psychology
Horseradish peroxidase
Horseradish Peroxidase - chemistry
Hydrogen Peroxide - analysis
Hydrogen Peroxide - chemistry
Microelectrodes
Nanotechnology - instrumentation
Nanotubes - chemistry
Nanotubes - ultrastructure
Nanowire array electrode
Reproducibility of Results
Self-assembled monolayer
Sensitivity and Specificity
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Summary:Vertically aligned nanowire array electrodes (NAEs) were prepared by electrodeposition of gold into an anodic aluminium oxide membrane (AAM), providing an ordered three-dimensional (3D) matrix for immobilization of redox proteins. Third-generation H 2O 2 biosensors were prepared by covalent immobilization of horseradish peroxidase (HRP) on the self-assembled monolayer modified NAEs. Direct electron transfer and electrocatalytic performances of the HRP/NAEs with different nanowire lengths (deposition time of 2, 4 and 5 h) were investigated. Results showed that with longer nanowires, better performances were achieved. The HRP/NAE 5 h (5 h deposition time) exhibited remarkable sensitivity (45.86 μA mM −1 cm −2) towards H 2O 2 with a detection limit of 0.42 μM ( S/ N = 3), linearity up to 15 mM and a response time of 4 s. The ordered 3D gold nanowire array with high conductivity, excellent electron transfer capability and good biocompatibility proved promising for fabricating sensitive, selective, stable and mediator-free enzymatic biosensors.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2009.10.018