Cloning and characterization of acetylcholinesterase 1 genes from insecticide-resistant field populations of Liposcelis paeta Pearman (Psocoptera: Liposcelididae)

• Published in: Insect biochemistry and molecular biology 2010-05, Vol.40 (5), p.415-424
• Main Authors: Wu, Shuang, Li, Ming, Tang, Pei-An, Felton, Gary W., Wang, Jin-Jun
• Format: Article
• Language: English
• Subjects: Ace 1 gene; ace gene; acetylcholinesterase; Acetylcholinesterase - genetics; Acetylcholinesterase - metabolism; amino acid sequences; Animals; Cloning, Molecular; Comparative quantitative expression; cross resistance; defense mechanisms; Drug Resistance - drug effects; Drug Resistance - genetics; epigenetics; gene expression; Genes, Insect; genetic polymorphism; inheritance (genetics); Insect Proteins - genetics; Insect Proteins - metabolism; Insecta - enzymology; Insecta - genetics; Insecticide resistance; Insecticides - pharmacology; Liposcelididae; Liposcelis; Liposcelis paeta; Liposcelis paeta Pearman; mechanism of action; Molecular cloning; molecular sequence data; nucleotide sequences; organophosphorus insecticides; Organothiophosphates - pharmacology; Paraoxon - analogs & derivatives; Paraoxon - pharmacology; polymerase chain reaction; sequence analysis; storage insects
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/j.ibmb.2010.04.001

The psocid, Liposcelis paeta Pearman, is an increasingly important polyphagous pest of stored products worldwide. Intensive use of organophosphorous insecticides for pest control has facilitated resistance development in psocids in China. Three insecticide-resistant field populations of L. paeta were collected from Nanyang city of Henan Province (NY), and Wuzhou (WZ) and Hezhou (HZ) cities of Guangxi Province, China. Previous studies have shown that psocids have different susceptibilities to insecticides. In addition, their AChE susceptibilities to paraoxon-ethyl and demeton-S-methyl also differed from each other. Acetylcholinesterase 1, which is one of the major targets for organophosphate insecticides, has been fully cloned and sequenced from these populations of L. paeta. Comparison of both nucleotide and deduced amino acid sequences revealed nucleotide polymorphisms among L. paeta ace 1 genes from different populations, but none of these polymorphisms correspond to the active sites in AChE 1 from other insects. The results of comparative quantitative real-time PCR indicated that the relative expression level of HZ ace 1 gene was the highest among three populations, which was 1.20 and 1.02-fold higher than those of NY and WZ populations, respectively. This may due to an epigenetic inheritance phenomenon, which allows organisms to respond to a particular environment through changes in gene expression.